A High-Performance Liquid Chromatography:Chemiluminescence Method for Potential Determination of Vardenafil in Dietary Supplement

被引：18
作者：
Di, Youjun [1 ]
Zhao, Min [2 ]
Nie, Yingchun [1 ]
Wang, Fei [3 ]
Lv, Jiagen [2 ]
机构：
[1] Shaanxi Inst Educ, Dept Chem, Xian 710061, Peoples R China
[2] Shaanxi Normal Univ, Sch Chem & Mat Sci, Xian 710062, Peoples R China
[3] Xian Municipal Publ Secur Bur Yanta, Xian 710061, Peoples R China
来源：
JOURNAL OF AUTOMATED METHODS & MANAGEMENT IN CHEMISTRY | 2011年
关键词：
SYNTHETIC PHOSPHODIESTERASE-5 INHIBITORS; STRUCTURE ELUCIDATION; MASS-SPECTROMETRY; DESIGNER DRUG; ESI-MS; SILDENAFIL; TADALAFIL; PRODUCTS; TABLETS; PLASMA;
D O I：
10.1155/2011/982186
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
A flow method of high-performance liquid chromatography (HPLC) seperation and chemiluminescence (CL) detection for sensitive vardenafil analysis in dietary supplements was developed. The vardenafil separation was achieved on a C18 column at 30 degrees C using ethanol-H3PO4 and ethylenediaminetetraacetic acid disodium salt (Na(2)EDTA) aqueous solution (25:75, v/v%) as mobile phase. The followed continuous CL detection was conducted based on the strong CL enhancement by the presence of vardenafil to luminol-K3Fe(CN)(6) reaction in alkaline medium. At the flow rate of 0.8 mL/min, the vardenafil retention time (t(R)) was 6.4 min. Factors that affected the HPLC resolution and CL detection were studied and optimized. The calibration curve obtained for vardenafil standard was linear in concentration range of 8.0 x 10(-7) similar to 1.0 x 10(-4) mol/L. The relative standard deviations (RSD) of intraday and interday precision were less than 3.5%. The proposed method was applied to the vardenafil determination in oral liquid, wine, and capsule samples.
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页数：6
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