Utilization of an evaporative light scattering high-performance size-exclusion chromatography acid oligomers

被引：8

作者：

Cameron, RG

Hotchkiss, AT

Kauffman, SW

Grohmann, K

机构：

[1] USDA, ARS, SAA, Citrus & Subtrop Prod Lab, Winter Haven, FL 33881 USA

[2] USDA, ARS, NAA, Eastern Reg Res Ctr, Wyndmoor, PA USA

来源：

JOURNAL OF CHROMATOGRAPHY A | 2003年 / 1011卷 / 1-2期

关键词：

galacturonic acid; polygalacturonic acid; pectin; carbohydrates;

D O I：

10.1016/S0021-9673(03)01171-3

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A high-performance size-exclusion chromatography-evaporative light scattering detector method was used to separate, detect and quantify galacturonic acid (GA) oligomers. In 40 mM acetic acid GA monomer, dimer and trimer could be separated with baseline resolution but polygalacturonic acid (PGA) precipitated and could not be eluted from the column. An NH4OAc, pH 3.7, buffer was developed as the eluent which separated GA oligomers as well as PGA and pectin without precipitation. Linear calibration curves for mono-, di- and tri-GA were produced with this buffer which could be used to estimate masses of tetra-, penta- and hexa-GA, as well as 19mer and 20mer. (C) 2003 Elsevier B.V. All rights reserved.

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页码：227 / 231

页数：5

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