Exosomal miR-1298 and lncRNA-RP11-583F2.2 Expression in Hepato-cellular Carcinoma

被引：29
作者：
Matboli, Marwa [1 ]
Labib, Mariam E. [1 ]
Nasser, Hanaa El-Tayeb [1 ]
El-Tawdi, Ahmed H. F. [2 ]
Habib, Eman K. [3 ]
Ali-Labib, Randa [1 ]
机构：
[1] Ain Shams Univ, Fac Med, Med Biochem & Mol Biol Dept, Cairo 11381, Egypt
[2] Mil Med Acad, Gen & Plast Surg Dept, Cairo, Egypt
[3] Ain Shams Univ, Fac Med, Anat Dept, Cairo, Egypt
来源：
CURRENT GENOMICS | 2020年 / 21卷 / 01期
关键词：
Hepatocellular carcinoma; exosome; miRNA; lncRNA; diagnosis; chronic hepatitis C; CANCER; BIOMARKER; PROGRESSION; BIOGENESIS; MICRORNAS; SECRETION; AUTOPHAGY; CELLS;
D O I：
10.2174/1389202920666191210111849
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Aim: The aim of this study was to explore the expression of exosomal non-coding RNAs (ncRNAs) in the sera of patients with HCC versus control. Methods: Firstly, Bioinformatics analysis was conducted to retrieve ncRNAs specific to HCC (hsamiRNA-1298 and lncRNA-RP11-583F2.2). Afterwards, extraction and characterization of exosomes were performed. We measured the expression of the chosen exosomal RNAs by reverse transcriptase quantitative real-time PCR in sera of 60 patients with HCC, 42 patients with chronic hepatitis C (CHC) infection and 18 healthy normal volunteers. Results: The exosomal ncRNAs [hsa-miRNA-1298, lncRNA-RP11-583F2.2] had better sensitivity and specificity than alpha-fetoprotein (AFP) in HCC diagnosis. Conclusion: The exosomal hsa-miRNA-1298, lncRNA-RP11-583F2.2 can be potential biomarkers for HCC diagnosis.
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页码：46 / 55
页数：10
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