Exosomal miR-29b found in aqueous humour mediates calcium signaling in diabetic patients with cataract

被引：13

作者：

Gao, Chao ^{[1
,2
]}

Liu, Xin ^{[2
,3
,4
,5
,6
]}

Fan, Fan ^{[2
,3
,4
,5
,6
]}

Yang, Jia-Ning ^{[2
,3
,4
,5
,6
]}

Zhou, Xi-Yue ^{[2
,3
,4
,5
,6
]}

Mei, Heng-Jun ^{[2
,3
,4
,5
,6
]}

Lin, Xiao-Lei ^{[2
,3
,4
,5
,6
]}

Luo, Yi ^{[2
,3
,4
,5
,6
]}

机构：

[1] Shihezi Univ, Sch Med, Affiliated Hosp 1, Shihezi 832000, Xinjiang Uygur, Peoples R China

[2] Fudan Univ, Coll Med, Eye & ENT Hosp, Eye Inst, Shanghai 200031, Peoples R China

[3] Fudan Univ, Shanghai Med Coll, State Key Lab Med Neurobiol, Inst Brain Sci, Shanghai 200031, Peoples R China

[4] Fudan Univ, Shanghai Med Coll, State Key Lab Med Neurobiol, Collaborat Innovat Ctr Brain Sci, Shanghai 200031, Peoples R China

[5] Sci & Technol Commiss Shanghai Municipal, Shanghai Key Lab Visual Impairment & Restorat, Shanghai 200031, Peoples R China

[6] Fudan Univ, Chinese Acad Med Sci, Natl Hlth Commiss, Key Lab Myopia, Shanghai 200031, Peoples R China

来源：

INTERNATIONAL JOURNAL OF OPHTHALMOLOGY | 2021年 / 14卷 / 10期

基金：

中国国家自然科学基金;

关键词：

exosomes; miR-29b; diabetes and cataracts; Ca2+; CACNA1C; EXTRACELLULAR VESICLES; ANGIOGENESIS; EXPRESSION; CYTOKINES; RATS; LENS;

D O I：

10.18240/ijo.2021.10.03

中图分类号：

R77 [眼科学];

学科分类号：

100212 ;

摘要：

AIM: To investigate the role of exosomal miR-29b and Ca2+ in regulating the function of human lens epithelial cells (HLECs). METHODS: Exosomes were isolated from human aqueous humour (AH) by ultracentrifugation, and visualized by nanoparticle tracking and transmission electron microscopy. Exosomal miRNA sequencing was performed to identify differentially expressed miRNAs between diabetes with cataracts (DMC) group and age-related cataracts (ARC) group. TargetScan was used to predict potential target of certain miRNA. The expression of CACNA1C mRNA was determined by quantitative real-time polymerase chain reaction and CACNA1C protein was determined by Western blotting. Concentration of Ca2+ in human AH and the culture supernatant of cells were detected by the calcium assay kit. Cell counting kit-8 was used to determine cell viability. RESULTS: Exosomes were isolated from human AH, which had a typical cup-shaped phenotype and a particle size distribution in accordance with micro extracellular vesicles. Exosomal miRNA sequencing revealed that miR- 29b was significantly downregulated in DMC group compared with ARC. Ca2+ concentration of human AH in DMC was higher than that in ARC. The culture supernatant of cells transfected with miR-29b inhibitors had a higher concentration of Ca2+ than that transfected with miR29b mimics. miR-29b reduced the viability of HLECs by upregulating CACNA1C expression. CONCLUSION: Exosomes isolated from human AH contains abundant miRNAs. A significantly expressed miRNA, miR-29b, can affect the concentration of Ca2+ and regulate HLEC processes by upregulating CACNA1C.

引用

页码：1484 / 1491

页数：8

共 42 条

[41] miR-203b: a novel regulator of MyoD expression in tilapia skeletal muscle [J].