ccrABEnt serine recombinase genes are widely distributed in the Enterococcus faecium and Enterococcus casseliflavus species groups and are expressed in E. faecium

被引:9
作者
Bjorkeng, Eva Katrin [1 ]
Tessema, Girum Tadesse [1 ]
Lundblad, Eirik Wasmuth [2 ]
Butaye, Patrick [3 ,4 ]
Willems, Rob [5 ]
Sollid, Johanna Ericsson [1 ]
Sundsfjord, Arnfinn [1 ,2 ]
Hegstad, Kristin [1 ,2 ]
机构
[1] Univ Tromso, Res Grp Host Microbe Interact, Dept Med Biol, Fac Hlth Sci, N-9037 Tromso, Norway
[2] Univ Hosp N Norway, Ref Ctr Detect Antimicrobial Resistance Res K, Dept Microbiol & Infect Control, N-9038 Tromso, Norway
[3] CODA CERVA VAR, Brussels, Belgium
[4] Univ Ghent, Dept Pathol Bacteriol & Poultry Dis, Fac Vet Med, B-9820 Merelbeke, Belgium
[5] Univ Med Ctr Utrecht, Dept Med Microbiol, NL-3584 CX Utrecht, Netherlands
来源
MICROBIOLOGY-SGM | 2010年 / 156卷
关键词
CASSETTE CHROMOSOME MEC; RESISTANT STAPHYLOCOCCUS-AUREUS; IDENTIFICATION; STRAINS; ELEMENT; DRIVEN; PCR;
D O I
10.1099/mic.0.041491-0
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The presence, distribution and expression of cassette chromosome recombinase (ccr) genes, which are homologous to the staphylococcal ccrAB genes and are designated ccrAB(Ent) genes, were examined in enterococcal isolates (n=421) representing 13 different species. A total of 118 (28%) isolates were positive for ccrAB(Ent) genes by PCR, and a number of these were confirmed by Southern hybridization with a ccrA(Ent) probe (n=76) and partial DNA sequencing of ccrA(Ent) and ccrB(Ent) genes (n=38). ccrAB(Ent) genes were present in Enterococcus faecium (58/216, 27%), Enterococcus durans (31/38, 82%), Enterococcus hirae (27/52, 50%), Enterococcus casseliflavus (1/4, 25%) and Enterococcus gallinarum (1/2, 50%). In the eight other species tested, including Enterococcus faecalis (n=94), ccrAB(Ent) genes were not found. Thirty-eight sequenced ccrAB(Ent) genes from five different enterococcal species showed 94-100% nucleotide sequence identity and linkage PCRs showed heterogeneity in the ccrAB(Ent) flanking chromosomal genes. Expression analysis of ccrAB(Ent) genes from the E. faecium DO strain showed constitutive expression as a bicistronic mRNA. The ccrAB(Ent) mRNA levels were lower during log phase than stationary phase in relation to total mRNA. Multi locus sequence typing was performed on 39 isolates. ccrABEnt genes were detected in both hospital-related (10/29, 34%) and non-hospital (4/10, 40%) strains of E. faecium. Various sequence types were represented by both ccrAB(Ent) positive and negative isolates, suggesting acquisition or loss of ccrAB(Ent) in E. faecium. In summary, ccrAB(Ent), genes, potentially involved in genome plasticity, are expressed in E. faecium and are widely distributed in the E. faecium and E. casseliflavus species groups.
引用
收藏
页码:3624 / 3634
页数:11
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