Enhanced production of microalgal lipids using a heterotrophic marine microalga Thraustochytrium sp. BM2

被引：30

作者：

Chen, Chun-Yen ^{[1
]}

Lee, Meng-Hsiu ^{[2
]}

Dong, Cheng-Di ^{[3
]}

Leong, Yoong Kit ^{[4
]}

Chang, Jo-Shu ^{[2
,4
,5
]}

机构：

[1] Natl Cheng Kung Univ, Univ Ctr Biosci & Biotechnol, Tainan, Taiwan

[2] Natl Cheng Kung Univ, Dept Chem Engn, Tainan, Taiwan

[3] Natl Kaohsiung Univ Sci & Technol, Dept Marine Environm Engn, Kaohsiung, Taiwan

[4] Tunghai Univ, Coll Engn, Dept Chem & Mat Engn, Taichung, Taiwan

[5] Natl Cheng Kung Univ, Res Ctr Energy Technol & Strategy, Tainan, Taiwan

来源：

BIOCHEMICAL ENGINEERING JOURNAL | 2020年 / 154卷

关键词：

Heterotrophic cultivation; Thrasutochytrium sp; Microalgal lipids; Glycerol; Corn steep liquor; DOCOSAHEXAENOIC ACID PRODUCTION; BIODIESEL PRODUCTION; FATTY-ACID; ENGINEERING STRATEGIES; CRUDE GLYCEROL; DHA PRODUCTION; WASTE-WATER; BIOMASS; GROWTH; OPTIMIZATION;

D O I：

10.1016/j.bej.2019.107429

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

In this study, the potential of Thraustochytrium sp. BM2 for cost-effective lipid production was investigated. Lipid content and productivity of BM2 reached 76% and 37 mg/L/h when corn steep liquor (12.5 g/L) and glycerol (10 g/L) was used as nitrogen and carbon source respectively. Supplementing an optimal amount of sea salt (2% w/v) further enhanced the lipid content to 79%. The inoculum size and age were also evaluated for their effect on lipid accumulation. A 48-h pre-culture at 0.15 g/L loading further improved the lipid productivity to 43.86 mg/L/h (or 1.052 g/L/d), which is a 340% increase when compared with the control test. Thus, Thraustochytrium sp. BM2 strain could serve as a low-cost and high-lipid-yield heterotrophic lipid producer using glycerol (a by-product of biodiesel manufacturing process) as carbon source and an inexpensive nitrogen source (i.e., corn steep liquor).

引用

页数：8

共 43 条

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