Three-dimensional stimulation and imaging-based functional optical microscopy of biological cells

被引：45

作者：

Quan, Xiangyu ^{[1
]}

Kumar, Manoj ^{[1
]}

Matoba, Osamu ^{[1
]}

Awatsuji, Yasuhiro ^{[2
]}

Hayasaki, Yoshio ^{[3
]}

Hasegawa, Satoshi ^{[3
]}

Wake, Hiroaki ^{[4
]}

机构：

[1] Kobe Univ, Grad Sch Syst Informat, Nada Ku, Rokkodai 1-1, Kobe, Hyogo 6578501, Japan

[2] Kyoto Inst Technol, Fac Elect Engn & Elect, Sakyo Ku, Kyoto 6068585, Japan

[3] Utsunomiya Univ, CORE, 7-1-2 Yoto, Utsunomiya, Tochigi 3218585, Japan

[4] Kobe Univ, Grad Sch Med, Div Syst Neurosci, Chuo Ku, Kusunoki Cho 7-5-1, Kobe, Hyogo 6500017, Japan

来源：

OPTICS LETTERS | 2018年 / 43卷 / 21期

基金：

日本学术振兴会;

关键词：

INCOHERENT DIGITAL HOLOGRAPHY; SPATIAL LIGHT-MODULATOR; OPTOGENETICS; SYSTEMS;

D O I：

10.1364/OL.43.005447

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

A new type of functional optical microscope system called three-dimensional (3D) stimulation and imaging-based functional optical microscopy (SIFOM) is proposed, to the best of our knowledge. SIFOM can precisely stimulate user-defined targeted biological cells and can simultaneously record the volumetric fluorescence distribution in a single acquisition. Precise and simultaneous stimulation of fluorescent-labeled biological cells is achieved by multiple 3D spots generated by digital holograms displayed on a phase-mode spatial light modulator. Single-shot 3D acquisition of the fluorescence distribution is accomplished by common-path off-axis incoherent digital holographic microscopy in which a diffraction grating with a focusing lens is displayed on another phase-mode spatial light modulator. The effectiveness of the proposed functional microscope system was verified in experiments using fluorescent microbeads and human lung cancer cells located at various defocused positions. The system can be used for manipulating the states of cells in optogenetics. (C) 2018 Optical Society of America

引用

页码：5447 / 5450

页数：4

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