Transcriptional and chromatin-based partitioning mechanisms from cell size

被引：34

作者：

Swaffer, Matthew P. ^{[1
]}

Kim, Jacob ^{[1
,2
]}

Chandler-Brown, Devon ^{[1
]}

Langhinrichs, Maurice ^{[1
]}

Marinov, Georgi K. ^{[3
]}

Greenleaf, William J. ^{[3
]}

Kundaje, Anshul ^{[3
]}

Schmoller, Kurt M. ^{[1
,4
]}

Skotheim, Jan M. ^{[1
]}

机构：

[1] Stanford Univ, Dept Biol, Stanford, CA 94305 USA

[2] Stanford Univ, Dept Chem & Syst Biol, Stanford, CA 94305 USA

[3] Stanford Univ, Dept Genet, Stanford, CA 94305 USA

[4] Helmholtz Zentrum Munchen, Inst Funct Epigenet, D-85764 Neuherberg, Germany

来源：

MOLECULAR CELL | 2021年 / 81卷 / 23期

关键词：

GENE-EXPRESSION; BUDDING-YEAST; SACCHAROMYCES-CEREVISIAE; PROTEIN LOCALIZATION; G1/S TRANSCRIPTION; CYCLE CONTROL; RNA-SYNTHESIS; COPY NUMBER; GROWTH; IDENTIFICATION;

D O I：

10.1016/j.molcel.2021.10.007

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Biosynthesis scales with cell size such that protein concentrations generally remain constant as cells grow. As an exception, synthesis of the cell-cycle inhibitor Whi5 "sub-scales"with cell size so that its concentration is lower in larger cells to promote cell-cycle entry. Here, we find that transcriptional control uncouples Whi5 synthesis from cell size, and we identify histones as the major class of sub-scaling transcripts besides WHI5 by screening for similar genes. Histone synthesis is thereby matched to genome content rather than cell size. Such sub-scaling proteins are challenged by asymmetric cell division because proteins are typically partitioned in proportion to newborn cell volume. To avoid this fate, Whi5 uses chromatin-binding to partition similar protein amounts to each newborn cell regardless of cell size. Disrupting both Whi5 synthesis and chromatin-based partitioning weakens G1 size control. Thus, specific transcriptional and partitioning mechanisms determine protein sub-scaling to control cell size.

引用

页码：4861 / +

页数：23