An efficient micropropagation protocol for Metasequoia glyptostroboides Hu et Cheng from shoot segments of 2-year-old trees

被引：13

作者：

Xiong, Yuping ^{[1
,2
]}

Chen, Shuangyan ^{[1
]}

Guo, Beiyi ^{[1
,2
]}

Niu, Meiyun ^{[1
,2
]}

Zhang, Xinhua ^{[1
]}

Li, Yuan ^{[1
]}

Wu, Kunlin ^{[1
]}

Zheng, Feng ^{[1
]}

Teixeira da Silva, Jaime A. ^{[3
]}

Zeng, Songjun ^{[1
]}

Ma, Guohua ^{[1
]}

机构：

[1] Chinese Acad Sci, South China Bot Garden, Guangdong Prov Key Lab Appl Bot, Guangzhou 510650, Peoples R China

[2] Univ Chinese Acad Sci, Beijing 100039, Peoples R China

[3] POB 7,Miki Cho PO,Ikenobe 3011-2, Miki, Kagawa 7610799, Japan

来源：

TREES-STRUCTURE AND FUNCTION | 2020年 / 34卷 / 01期

关键词：

Acclimatization; Dawn redwood; Conifer; Regeneration; Root induction; ORGANOGENESIS;

D O I：

10.1007/s00468-019-01905-7

中图分类号：

S7 [林业];

学科分类号：

0829 ; 0907 ;

摘要：

Metasequoia glyptostroboides Hu et Cheng (dawn redwood), a known relict living fossil, is listed as a Class I rare and endangered tree in China. An efficient in vitro propagation protocol was established in this study to overcome the shortage of germplasm that cannot be compensated by conventional reproduction and as a way to preserve this tree. This objective was achieved by testing the effect of plant growth regulators (PGRs) on shoot propagation and root formation. Axillary shoots, which could be induced on PGR-free woody plant medium (WPM), were efficiently propagated on WPM supplemented with 16.0 mu M zeatin and 2.5 mu M indole-3-butyric acid. Improved rooting was possible on WPM supplemented with 0.05 mu M alpha-naphthaleneacetic acid. Rooting was possible both in vitro in vermiculite-based PGR-free liquid culture and ex vitro in plastic bags containing perlite: vermiculite (1:1, v/v). This effective in vitro micropropagation protocol for M. glyptostroboides serves as a way to conserve this tree and provides an opportunity for mass production to alleviate strain on natural populations.

引用

页码：307 / 313

页数：7