Pressure effects on ligand binding kinetics and the heme environment in myoglobin

被引：0

作者：

Schulte, A ^{[1
]}

Galkin, O ^{[1
]}

机构：

[1] Univ Cent Florida, Dept Phys, Orlando, FL 32816 USA

来源：

ADVANCES IN HIGH PRESSURE BIOSCIENCE AND BIOTECHNOLOGY II, PROCEEDINGS | 2003年

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D O I：

暂无

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The multistep kinetics of ligand binding to myoglobin is investigated at variable pressure (0.1 - 190 MPa) and temperature (180 - 300 K). The amplitude of the geminate rebinding process increases with pressure corresponding to a smaller escape fraction of ligands into the solvent. High pressures may alter the accessibility of internal cavities in the protein and specific ligand pathways.

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页码：121 / 124

页数：4

相关论文

共 13 条

[1]

ADACHI S, 1989, J BIOL CHEM, V264, P18896

[2] DYNAMICS OF LIGAND-BINDING TO MYOGLOBIN [J].

AUSTIN, RH ;

BEESON, KW ;

EISENSTEIN, L ;

FRAUENFELDER, H ;

GUNSALUS, IC .

BIOCHEMISTRY, 1975, 14 (24) :5355-5373

[3] Structure of a ligand-binding intermediate in wild-type carbonmonoxy myoglobin [J].

Chu, K ;

Vojtchovsky, J ;

McMahon, BH ;

Sweet, RM ;

Berendzen, J ;

Schlichting, I .

NATURE, 2000, 403 (6772) :921-923

[4]

Doster W, 2001, TRENDS HIGH PRESSURE

[5] PROTEINS AND PRESSURE [J].

FRAUENFELDER, H ;

ALBERDING, NA ;

ANSARI, A ;

BRAUNSTEIN, D ;

COWEN, BR ;

HONG, MK ;

IBEN, IET ;

JOHNSON, JB ;

LUCK, S ;

MARDEN, MC ;

MOURANT, JR ;

ORMOS, P ;

REINISCH, L ;

SCHOLL, R ;

SCHULTE, A ;

SHYAMSUNDER, E ;

SORENSEN, LB ;

STEINBACH, PJ ;

XIE, AH ;

YOUNG, RD ;

YUE, KT .

JOURNAL OF PHYSICAL CHEMISTRY, 1990, 94 (03) :1024-1037

[6] Pressure effects on the proximal heme pocket in myoglobin probed by Raman and near-infrared absorption spectroscopy [J].

Galkin, O ;

Buchter, S ;

Tabirian, A ;

Schulte, A .

BIOPHYSICAL JOURNAL, 1997, 73 (05) :2752-2763

[7] Cytochrome P-450-CO and substrates:: lessons from ligand binding under high pressure [J].

Jung, C .

BIOCHIMICA ET BIOPHYSICA ACTA-PROTEIN STRUCTURE AND MOLECULAR ENZYMOLOGY, 2002, 1595 (1-2) :309-328

[8] Solvent composition and viscosity effects on the kinetics of CO binding to horse myoglobin [J].

Kleinert, T ;

Doster, W ;

Leyser, H ;

Petry, W ;

Schwarz, V ;

Settles, M .

BIOCHEMISTRY, 1998, 37 (02) :717-733

[9]

KTICHEN DB, 1992, BIOCHEMISTRY-US, V31, P10083

[10] ACTIVATION VOLUME AND ENERGETIC PROPERTIES OF THE BINDING OF CO TO HEMOPROTEINS [J].

LANGE, R ;

HEIBERLANGER, I ;

BONFILS, C ;

FABRE, I ;

NEGISHI, M ;

BALNY, C .

BIOPHYSICAL JOURNAL, 1994, 66 (01) :89-98