Human Plasma Membrane-Derived Vesicles Halt Proliferation and Induce Differentiation of THP-1 Acute Monocytic Leukemia Cells

被引：48

作者：

Ansa-Addo, Ephraim A. ^{[1
]}

Lange, Sigrun ^{[2
]}

Stratton, Dan ^{[1
]}

Antwi-Baffour, Samuel ^{[1
]}

Cestari, Igor ^{[1
,4
]}

Ramirez, Marcel I. ^{[1
,4
]}

McCrossan, Maria V. ^{[3
]}

Inal, Jameel M. ^{[1
]}

机构：

[1] London Metropolitan Univ, Cellular & Mol Immunol Res Ctr, Sch Human Sci, Fac Life Sci, London N7 8DB, England

[2] UCL, Inst Womens Hlth Maternal & Fetal Med, Perinatal Brain Repair Grp, London WC1E 6BT, England

[3] London Sch Hyg & Trop Med, Immunol Unit, London WC1, England

[4] Inst Oswaldo Cruz Fiocruz, Rio De Janeiro, Brazil

来源：

JOURNAL OF IMMUNOLOGY | 2010年 / 185卷 / 09期

关键词：

GROWTH-FACTOR-BETA; MIGRATION INHIBITORY FACTOR; REGULATORY T-CELLS; VITAMIN-D-RECEPTOR; TGF-BETA; MESSENGER-RNA; MICROVESICLES; MICROPARTICLES; MECHANISMS; RESISTANCE;

D O I：

10.4049/jimmunol.1001656

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Plasma membrane-derived vesicles (PMVs) are small intact vesicles released from the cell surface that play a role in intercellular communication. We have examined the role of PMVs in the terminal differentiation of monocytes. The myeloid-differentiating agents all-trans retinoic acid/PMA and histamine, the inflammatory mediator that inhibits promonocyte proliferation, induced an intracellular Ca(2+)-mediated PMV (as opposed to exosome) release from THP-1 promonocytes. These PMVs cause THP-1 cells to enter G(0)-G(1) cell cycle arrest and induce terminal monocyte-to-macrophage differentiation. Use of the TGF-beta receptor antagonist SB-431542 and anti-TGF-beta 1 Ab showed that this was due to TGF-beta 1 carried on PMVs. Although TGF-beta 1 levels have been shown to increase in cell culture supernatants during macrophage differentiation and dendritic cell maturation, the presence of TGF-beta 1 in PMVs is yet to be reported. In this study, to our knowledge we show for the first time that TGF-beta 1 is carried on the surface of PMVs, and we confirm the presence within PMVs of certain leaderless proteins, with reported roles in myeloid cell differentiation. Our in vitro findings support a model in which TGF-beta 1-bearing PMVs, released from promonocytic leukemia cells (THP-1) or primary peripheral blood monocytes on exposure to sublytic complement or after treatment with a differentiation therapy agent, such as all-trans retinoic acid, significantly reduce proliferation of THP-1 cells. Such PMVs also induce the terminal differentiation of primary peripheral blood monocytes as well as THP-1 monocytes. The Journal of Immunology, 2010, 185: 5236-5246.

引用

页码：5236 / 5246

页数：11

共 43 条

[11] Regulated secretion of macrophage migration inhibitory factor is mediated by a non-classical pathway involving an ABC transporter [J].

Flieger, O ;

Engling, A ;

Bucala, R ;

Lue, HQ ;

Nickel, W ;

Bernhagen, J .

FEBS LETTERS, 2003, 551 (1-3) :78-86

[12] Induction of microRNAs, mir-155, mir-222, mir-424 and mir-503, promotes monocytic differentiation through combinatorial regulation [J].

Forrest, A. R. R. ;

Kanamori-Katayama, M. ;

Tomaru, Y. ;

Lassmann, T. ;

Ninomiya, N. ;

Takahashi, Y. ;

de Hoon, M. J. L. ;

Kubosaki, A. ;

Kaiho, A. ;

Suzuki, M. ;

Yasuda, J. ;

Kawai, J. ;

Hayashizaki, Y. ;

Hume, D. A. ;

Suzuki, H. .

LEUKEMIA, 2010, 24 (02) :460-466

[13] Resistance to 1,25D-induced differential in human acute myeloid leukemia HL60-40AF cells is associated with reduced transcriptional activity and nuclear localization of the vitamin D receptor [J].

Garay, Edward ;

Donnelly, Robert ;

Wang, Xuening ;

Studzinski, George P. .

JOURNAL OF CELLULAR PHYSIOLOGY, 2007, 213 (03) :816-825

[14] The platelet microparticle proteome [J].

Garcia, BA ;

Smalley, DM ;

Cho, HJ ;

Shabanowitz, J ;

Ley, M ;

Hunt, DF .

JOURNAL OF PROTEOME RESEARCH, 2005, 4 (05) :1516-1521

[15] Activated polymorphonuclear neutrophils disseminate anti-inflammatory microparticles by ectocytosis [J].

Gasser, O ;

Schifferli, JA .

BLOOD, 2004, 104 (08) :2543-2548

[16] Macrophage migration inhibitory factor induces macrophage recruitment via CC chemokine ligand 2 [J].

Gregory, Julia L. ;

Morand, Eric F. ;

McKeown, Sonja J. ;

Ralph, Jennifer A. ;

Hall, Pamela ;

Yang, Yuan H. ;

McColl, Shaun R. ;

Hickey, Michael J. .

JOURNAL OF IMMUNOLOGY, 2006, 177 (11) :8072-8079

[17] A specific inhibitor of TGF-β receptor kinase, SB-431542, as a potent antitumor agent for human cancers [J].

Halder, SK ;

Beauchamp, RD ;

Datta, PK .

NEOPLASIA, 2005, 7 (05) :509-521

[18] 1α,25-dihydroxyvitamin D3-induced myeloid cell differentiation is regulated by a vitamin D receptor-phosphatidylinositol 3-kinase signaling complex [J].

Hmama, Z ;

Nandan, D ;

Sly, L ;

Knutson, KL ;

Herrera-Velit, P ;

Reiner, NE .

JOURNAL OF EXPERIMENTAL MEDICINE, 1999, 190 (11) :1583-1594

[19] Membrane microparticles:: Two sides of the coin [J].

Hugel, B ;

Carmen, M ;

Martínez, MC ;

Kunzelmann, C ;

Freyssinet, JM .

PHYSIOLOGY, 2005, 20 :22-27

[20] Detection of microRNA Expression in Human Peripheral Blood Microvesicles [J].

Hunter, Melissa Piper ;

Ismail, Noura ;

Zhang, Xiaoli ;

Aguda, Baltazar D. ;

Lee, Eun Joo ;

Yu, Lianbo ;

Xiao, Tao ;

Schafer, Jeffrey ;

Lee, Mei-Ling Ting ;

Schmittgen, Thomas D. ;

Nana-Sinkam, S. Patrick ;

Jarjoura, David ;

Marsh, Clay B. .

PLOS ONE, 2008, 3 (11)

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