Electromembrane Extraction of Posaconazole for Matrix-Assisted Laser Desorption/Ionization Mass Spectrometric Detection

被引:1
作者
Chen, Chi-Sheng [1 ]
Chen, Wen-Chi [2 ,3 ,4 ]
Chang, Sarah Y. [1 ]
机构
[1] Natl Kaohsiung Normal Univ, Dept Chem, 62 Shenjhong Rd, Kaohsiung 824, Taiwan
[2] Kaohsiung Vet Gen Hosp, Dept Med, Div Gastroenterol & Hepatol, 386 Ta Chung 1st Rd, Kaohsiung 813, Taiwan
[3] Natl Yang Ming Chiao Tung Univ, Fac Med, Sch Med, Taipei 112, Taiwan
[4] Natl Sun Yat Sen Univ, Coll Sci, Inst Biomed Sci, Kaohsiung 804, Taiwan
关键词
electromembrane extraction; matrix-assisted laser desorption; ionization mass spectrometry; basic drug; posaconazole; LIQUID-LIQUID MICROEXTRACTION; POLYMER INCLUSION MEMBRANES; HUMAN PLASMA; ANTIFUNGAL DRUGS; TRIAZOLE; PERFORMANCE; SAMPLES;
D O I
10.3390/membranes12060620
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
A new mode of electromembrane extraction (EME) has been developed for detection via matrix-assisted laser desorption/ionization mass spectrometry (MALDI/MS). Posaconazole, extracted from 8 mL of a 10 mM trifluoroacetic acid solution onto a thin polyvinylidene difluoride (PVDF) membrane, was used as a model analyte. The transport was forced by an electrical potential difference between two electrodes inside the lumen of a hollow fiber and glass tube. Under an application of 80 V, cationic posaconazole in the sample solution moved toward the negative electrode inside the glass tube and was trapped by the PVDF membrane on the side. After 15 min of extraction, 3 mu L of alpha-cyano-4-hydroxycinnamic acid (CHCA) solution was applied on top of the membrane, which was then analyzed by MALDI/MS. Under optimal extraction conditions, the calibration curve of posaconazole was linear over a concentration range of 0.10-100.00 nM. The limit of detection (LOD) at a signal-to-noise ratio of 3 was 0.03 nM with an enhancement factor of 138 for posaconazole. The application of this method to the determination of posaconazole in human serum samples was also successfully demonstrated.
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页数:11
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