Acute stress affects the global DNA methylation profile in rat brain: Modulation by physical exercise

被引：52

作者：

Rodrigues, Gelson M., Jr. ^{[1
]}

Toffoli, Leandro V. ^{[1
]}

Manfredo, Marcelo H. ^{[1
]}

Francis-Oliveira, Jose ^{[2
]}

Silva, Andrey S. ^{[2
]}

Raquel, Hiviny A. ^{[2
]}

Martins-Pinge, Marli C. ^{[2
]}

Moreira, Estefania G. ^{[2
]}

Fernandes, Karen B. ^{[1
]}

Pelosi, Gislaine G. ^{[2
]}

Gomes, Marcus V. ^{[1
]}

机构：

[1] Univ Norte Parana UNOPAR, Londrina, Brazil

[2] Univ Estadual Londrina, Londrina, Brazil

来源：

BEHAVIOURAL BRAIN RESEARCH | 2015年 / 279卷

关键词：

Acute stress; Epigenetics; DNA methylation; Physical exercise; Dnmt1; Bdnf; CARDIOVASCULAR-RESPONSES; EPIGENETIC REGULATION; PSYCHOLOGICAL STRESS; PERIAQUEDUCTAL GRAY; PREFRONTAL CORTEX; GENE-EXPRESSION; BDNF GENE; VULNERABILITY; EXPOSURE;

D O I：

10.1016/j.bbr.2014.11.023

中图分类号：

B84 [心理学]; C [社会科学总论]; Q98 [人类学];

学科分类号：

03 ; 0303 ; 030303 ; 04 ; 0402 ;

摘要：

The vulnerability of epigenetic marks of brain cells to environmental stimuli and its implication for health have been recently debated. Thus, we used the rat model of acute restraint stress (ARS) to evaluate the impact of stress on the global DNA methylation and on the expression of the Dnmt1 and Bdnf genes of hippocampus, cortex, hypothalamus and periaqueductal gray (PAG). Furthermore, we verified the potential of physical exercise to modulate epigenetic responses evoked by ARS. Sedentary male Wistar rats were submitted to ARS at the 75th postnatal day (PND), whereas animals from a physically active group were previously submitted to swimming sessions (35-74th PND) and to ARS at the 75th PND. Global DNA methylation profile was quantified using an ELISA-based method and the quantitative expression of the Dnmt1 and Bdnf genes was evaluated by real-time PCR. ARS induced a decrease in global DNA methylation in hippocampus, cortex and PAG of sedentary animals and an increased expression of Bdnf in PAG. No change in DNA methylation was associated with ARS in the exercised animals, although it was associated with abnormal expression of Dnmt1 and Bdnf in cortex, hypothalamus and PAG. Our data reveal that ARS evokes adaptive changes in global DNA methylation of rat brain that are independent of the expression of the Dnmt1 gene but might be linked to abnormal expression of the Bdnf gene in the PAG. Furthermore, our evidence indicates that physical exercise has the potential to modulate changes in DNA methylation and gene expression consequent to ARS. (C) 2014 Elsevier B.V. All rights reserved.

引用

页码：123 / 128

页数：6

共 37 条

[31] BDNF PRODUCES ANALGESIA IN THE FORMALIN TEST AND MODIFIES NEUROPEPTIDE LEVELS IN RAT-BRAIN AND SPINAL-CORD AREAS ASSOCIATED WITH NOCICEPTION [J].