Flavonoid Composition of Tarocco (Citrus sinensis L. Osbeck) Clone "Lempso" and Fast Antioxidant Activity Screening by DPPH-UHPLC-PDA-IT-TOF

被引：16

作者：

Sommella, Eduardo ^{[1
,2
]}

Pagano, Francesco ^{[1
,2
]}

Pepe, Giacomo ^{[1
,2
]}

Ostacolo, Carmine ^{[3
]}

Manfra, Michele ^{[4
]}

Chieppa, Marcello ^{[5
,6
]}

Di Sanzo, Rosa ^{[1
]}

Carabetta, Sonia ^{[1
]}

Campiglia, Pietro ^{[2
,6
]}

Russo, Mariateresa ^{[1
]}

机构：

[1] Univ Mediterranea Reggio Calabria, Food Chem Lab, Dipartimento Agr, Via Melissari,Stecca 4, I-89122 Reggio Di Calabria, Italy

[2] Univ Salerno, Sch Pharm, Dept Pharm, Via Giovanni Paolo 2 132, I-84084 Fisciano, SA, Italy

[3] Univ Napoli Federico II, Dept Pharm, Via D Montesano 49, I-80131 Naples, Italy

[4] Univ Basilicata, Dept Sci, Viale Ateneo Lucano 10, I-85100 Potenza, Italy

[5] Natl Inst Gastroenterol S de Bellis, Inst Res, Castellana Grotte, BA, Italy

[6] Ebris, Via De Renzi 50, I-84125 Salerno, Italy

来源：

PHYTOCHEMICAL ANALYSIS | 2017年 / 28卷 / 06期

关键词：

Tarocco Lempso; anthocyanins; flavonoids; antioxidant; DPPH-UHPLC-PDA; HPLC-DAD-TOF/MS; HORT. EX TAN; NATURAL ANTIOXIDANTS; IN-VITRO; JUICE; POLYPHENOLS; MECHANISMS; FLAVANONES; HESPERETIN; HESPERIDIN;

D O I：

10.1002/pca.2701

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Introduction - Clonal selection and hybridisation are valid strategies to obtain fruits with enhanced sensorial and nutraceutical properties. Within Citrus sinensis varieties, Tarocco clone "Lempso" is a typical product of the Calabria region (Italy) characterised by its red pulp. This is the first report concerning its accurate profiling. Objective - To characterise in detail the flavonoid composition of Lempso clone and to compare its antioxidant potential with other Citrus varieties by a fast screening method. Methodology - Extracts were subjected to solid phase extraction and the qualitative/quantitative profile was elucidated through ultra-high performance liquid chromatography (UHPLC) coupled to photodiode array (PDA) and ion trap time-of-flight (IT-TOF) mass spectrometry detection, and compared to both Cleopatra mandarin (Citrus reticulata) and blood orange (Citrus sinensis (L.) Osbeck) Sanguinello varieties. The antioxidant activity was assessed by pre-column 2,2-diphenyl-1-picrylhydrazyl (DPPH) reaction coupled to UHPLC-PDA. Results - Lempso is characterised by flavonoids (17) and anthocyanins (8). Flavanones content (Hesperidin: 57.19 +/- 0.49, Vicenin-2: 4.59 +/- 0.03, Narirutin: 5.78 +/- 0.13 mg/100 mL) was considerably higher than Cleopatra and Sanguinello varieties. The developed DPPH-UHPLC-PDA method provides information regarding the single contributions to antioxidant activity, highlighting how Ferulic acid, Quercetin and Cyanidin derivatives possess considerable radical scavenging activity (> 50%). The total antioxidant activity was also evaluated and compared with positive controls, showing higher scavenging activity than Cleopatra and Sanguinello (IC50: 333.76 +/- 10.81 mu g/mL vs. 452.62 +/- 10.81 and 568.39 +/- 26.98 mu g/mL, respectively). Conclusion - These data evidence the nutraceutical potential of Lempso variety, which could be an ingredient for functional beverages. Copyright (c) 2017 John Wiley & Sons, Ltd.

引用

页码：521 / 528

页数：8

共 33 条

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