Insights into the molecular basis of biocontrol of Brassica pathogens by Bacillus amyloliquefaciens UCMB5113 lipopeptides

被引:21
作者
Asari, Shashidar [1 ]
Ongena, Marc [2 ]
Debois, Delphine [3 ]
De Pauw, Edwin [3 ]
Chen, Kunling [1 ,4 ]
Bejai, Sarosh [1 ]
Meijer, Johan [1 ]
机构
[1] Swedish Univ Agr Sci, Dept Plant Biol, Uppsala Bioctr, Linnean Ctr Plant Biol, S-75007 Uppsala, Sweden
[2] Univ Liege Gembloux Agrobio Tech, Microbial Proc & Interact Lab, B-5030 Gembloux, Belgium
[3] Univ Liege, Mass Spectrometry Lab, B-4000 Liege, Belgium
[4] Chinese Acad Sci, Inst Genet & Dev Biol, Beijing 100101, Peoples R China
基金
瑞典研究理事会;
关键词
Antagonism; Arabidopsis thaliana; Bacillus amyloliquefaciens; beneficial bacteria; biocontrol; lipopeptide; rhizosphere; INDUCED SYSTEMIC RESISTANCE; PROMOTING RHIZOBACTERIA PGPR; ARABIDOPSIS-THALIANA; DEFENSE RESPONSES; PLANT-GROWTH; CYCLIC LIPOPEPTIDES; ACQUIRED-RESISTANCE; BOTRYTIS-CINEREA; SALICYLIC-ACID; SUBTILIS;
D O I
10.1093/aob/mcx089
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Background and Aims Certain micro-organisms can improve plant protection against pathogens. The protective effect may be direct, e.g. due to antibiotic compounds, or indirect, by priming of plant defence as induced systemic resistance (ISR). The plant growth-promoting rhizobacterium Bacillus amyloliquefaciens UCMB5113 shows potential for disease management of oilseed rape. To investigate the mode of action of this protection, especially in relation to jasmonic acid-dependent ISR, Bacillus UCMB5113 was tested with Arabidopsis thaliana mutants and several important fungal pathogens of Brassica species. Methods Secreted lipopeptide fractions from Bacillus UCMB5113, together with synthetic peptide mimics, were evaluated for their effects on fungal phytopathogens and A. thaliana. The structures of secreted lipopeptides were analysed using mass spectrometry. Plant mutants and reporter lines were used to identify signalling steps involved in disease suppression by lipopeptides. Key Results In plate tests Bacillus UCMB5113 and lipopeptide extracts suppressed growth of several fungal pathogens infecting Brassica plants. Separation of secreted lipopeptides using reversed-phase high-performance liquid chromatography revealed several fractions that inhibited fungal growth. Analysis by mass spectrometry identified the most potent compounds as novel linear forms of antifungal fengycins, with synthetic peptide mimics confirming the biological activity. Application of the lipopeptide extracts on Arabidopsis roots provided systemic protection against Alternaria brassicicola on leaves. Arabidopsis signalling mutants and PDF1.2 and VSP2 promoter-driven GUS lines indicated that the lipopeptide fraction involved jasmonic-acid-dependent host responses for suppression of fungal growth indicative of ISR. Conclusions The ability of Bacillus UCMB5113 to counteract pathogens using both antagonistic lipopeptides and through ISR provides a promising tool for sustainable crop production.
引用
收藏
页码:551 / 562
页数:12
相关论文
共 56 条
[1]   Improving Maternal and Newborn Health: Effectiveness of a Community Health Worker Program in Rural Kenya [J].
Adam, Mary B. ;
Dillmann, Maria ;
Chen, Mei-Kuang ;
Mbugua, Simon ;
Ndung'u, Joram ;
Mumbi, Priscilla ;
Waweru, Eunice ;
Meissner, Peter .
PLOS ONE, 2014, 9 (08)
[2]   ABA is an essential signal for plant resistance to pathogens affecting JA biosynthesis and the activation of defenses in Arabidopsis [J].
Adie, Bruce A. T. ;
Perez-Perez, Julian ;
Perez-Perez, Manuel M. ;
Godoy, Marta ;
Sanchez-Serrano, Jose-J. ;
Schmelz, Eric A. ;
Solano, Roberto .
PLANT CELL, 2007, 19 (05) :1665-1681
[3]   Genome mining: Prediction of lipopeptides and polyketides from Bacillus and related Firmicutes [J].
Aleti, Gajender ;
Sessitsch, Angela ;
Brader, Guenter .
COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL, 2015, 13 :192-203
[4]   The plant-associated Bacillus amyloliquefaciens strains MEP218 and ARP23 capable of producing the cyclic lipopeptides iturin or surfactin and fengycin are effective in biocontrol of sclerotinia stem rot disease [J].
Alvarez, F. ;
Castro, M. ;
Principe, A. ;
Borioli, G. ;
Fischer, S. ;
Mori, G. ;
Jofre, E. .
JOURNAL OF APPLIED MICROBIOLOGY, 2012, 112 (01) :159-174
[5]   Plant growth-promoting rhizobacteria (PGPR): Their potential as antagonists and biocontrol agents [J].
Beneduzi, Anelise ;
Ambrosini, Adriana ;
Passaglia, Luciane M. P. .
GENETICS AND MOLECULAR BIOLOGY, 2012, 35 (04) :1044-1051
[6]   Plant growth-promoting rhizobacteria (PGPR): emergence in agriculture [J].
Bhattacharyya, P. N. ;
Jha, D. K. .
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY, 2012, 28 (04) :1327-1350
[7]  
CAO H, 1994, PLANT CELL, V6, P1583, DOI 10.1105/tpc.6.11.1583
[8]   Plant Defense Stimulation by Natural Isolates of Bacillus Depends on Efficient Surfactin Production [J].
Cawoy, Helene ;
Mariutto, Martin ;
Henry, Guillaume ;
Fisher, Christophe ;
Vasilyeva, Natallia ;
Thonart, Philippe ;
Dommes, Jacques ;
Ongena, Marc .
MOLECULAR PLANT-MICROBE INTERACTIONS, 2014, 27 (02) :87-100
[9]  
Cawoy H, 2011, PESTICIDES IN THE MODERN WORLD - PESTICIDES USE AND MANAGEMENT, P273
[10]   More than Anticipated - Production of Antibiotics and Other Secondary Metabolites by Bacillus amyloliquefaciens FZB42 [J].
Chen, Xiao-Hua ;
Koumoutsi, Alexandra ;
Scholz, Romy ;
Borriss, Rainer .
JOURNAL OF MOLECULAR MICROBIOLOGY AND BIOTECHNOLOGY, 2009, 16 (1-2) :14-24