Two-photon excitation fluorescence microscopy and its application in functional connectomics

被引：18
作者：
Nemoto, Tomomi [1 ]
Kawakami, Ryosuke [1 ]
Hibi, Terumasa [1 ]
Iijima, Koichiro [1 ]
Otomo, Kohei [1 ]
机构：
[1] Hokkaido Univ, Res Inst Elect Sci, Lab Mol & Cellular Biophys, Kita Ku, Sapporo, Hokkaido 0010020, Japan
来源：
MICROSCOPY | 2015年 / 64卷 / 01期
基金：
日本科学技术振兴机构;
关键词：
non-linear optics; ultrashort pulse laser; in vivo imaging; neuronal activity; EXOCYTOSIS; NEURONS; MODELS; TISSUE; DEEP;
D O I：
10.1093/jmicro/dfu110
中图分类号：
TH742 [显微镜];
学科分类号：
摘要：
Two-photon excitation fluorescence microscopy has become widely used in various life science fields in this decade. In the field of neuroscience in particular, in vivo two-photon microscopy has provided vital information on neural activity and brain function. In the current era of connectomics, visualization of the morphology and activity of numerous neurons in ever larger regions of the living brain are required within short periods. Based on this viewpoint, we discuss the fundamentals, advantages and potential of two-photon excitation fluorescence microscopy for the investigation of neural circuit functions.
引用
收藏
页码：9 / 15
页数：7
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