Phase Separation and Nanodomain Formation in Hybrid Polymer/Lipid Vesicles

被引：71

作者：

Dao, T. P. Tuyen ^{[1
,2
,3
,4
]}

Fernandes, F. ^{[3
,4
]}

Er-Rafik, M. ^{[5
]}

Salva, R. ^{[1
,2
]}

Schmutz, M. ^{[5
]}

Brulet, A. ^{[6
]}

Prieto, M. ^{[3
,4
]}

Sandre, O. ^{[1
,2
]}

Le Meins, J. -F. ^{[1
,2
]}

机构：

[1] Univ Bordeaux, LCPO UMR 5629, F-33600 Pessac, France

[2] CNRS, Lab Chim Polymeres Organ, UMR 5629, F-33600 Pessac, France

[3] Univ Lisbon, Inst Super Tecn, Ctr Quim Fis Mol, P-1049001 Lisbon, Portugal

[4] Univ Lisbon, Inst Super Tecn, Inst Nanosci & Nanotechnol, P-1049001 Lisbon, Portugal

[5] Univ Strasbourg, UPR CNRS 22, Inst Charles Sadron, F-67034 Strasbourg, France

[6] CEA Saclay, CEA CNRS UMR12, Lab Leon Brillouin, F-91191 Gif Sur Yvette, France

来源：

ACS MACRO LETTERS | 2015年 / 4卷 / 02期

关键词：

MEMBRANE-COMPOSITION; LIPID-MEMBRANES; ENERGY-TRANSFER; LINE TENSION; RAFTS; DOMAINS; SIZE; MICELLES; SYSTEMS; BLENDS;

D O I：

10.1021/mz500748f

中图分类号：

O63 [高分子化学（高聚物）];

学科分类号：

070305 ; 080501 ; 081704 ;

摘要：

Hybrid polymer/lipid large unilamellar vesicles (LUVS) were studied by small angle neutron scattering (SANS), time-resolved Forster resonance energy transfer (TR-FRET), and cryo-transmission electron microscopy (cryo-TEM). For the first time in hybrid vesicles, evidence for phase separation at the nanoscale was obtained, leading to the formation of stable nanodomains enriched either in lipid or polymer. This stability was allowed by using vesicle-forming copolymer with a membrane thickness dose to the lipid bilayer thickness, thereby minimizing the hydrophobic mismatch at the domain periphery. Hybrid giant unilamellar vesicles (GUVs) with the same composition have been previously shown to be unstable and susceptible to fission, suggesting a role of curvature in the stabilization of nanodomains in these structures.

引用

页码：182 / 186

页数：5

共 30 条

[1] Cell biology - A role for lipid shells in targeting proteins to caveolae, rafts, and other lipid domains [J].