Polarization-resolved second-harmonic-generation imaging of photoaged dermal collagen fiber

被引:0
|
作者
Yasui, Takeshi [1 ]
Takahashi, Yu [1 ]
Araki, Tsutomu [1 ]
机构
[1] Osaka Univ, Grad Sch Engg Sci, Osaka 5608531, Japan
关键词
Second-harmonic-generation; microscopy; polarization; collagen; orientation; photoaging; wrinkle; HUMAN SKIN; MULTIPHOTON FLUORESCENCE; CONFOCAL MICROSCOPY; LASER-PULSES; HUMAN TISSUE; ORIENTATION; GENERATION; MOUSE;
D O I
10.1117/12.808672
中图分类号
TH742 [显微镜];
学科分类号
摘要
Polarization-resolved second-harmonic-generation (SHG) microscopy is useful for assessment of collagen fiber orientation in tissues. In this paper, we investigated the relation between wrinkle direction and collagen orientation in ultraviolet-B-exposed (UVB-exposed) skin using polarization-resolved SHG microscopy. A polarization anisotropic image of the SHG light indicated that wrinkle direction in UVB-exposed skin is predominantly parallel to the orientation of dermal collagen fibers whereas no-UVB-exposed skin was dominated by collagen orientation parallel to the meridian line of body. The method proposed has the potential to become a powerful non-invasive tool for assessment of cutaneous photoaging.
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页数:9
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