Reprogramming of Human Retinal Pigment Epithelial Cells under the Effect of bFGF In Vitro

被引：11

作者：

Shafei, E. V. ^{[1
]}

Kurinov, A. M. ^{[1
]}

Kuznetsova, A. V. ^{[1
]}

Aleksandrova, M. A. ^{[1
]}

机构：

[1] Russian Acad Sci, NK Koltsov Inst Dev Biol, Moscow, Russia

来源：

BULLETIN OF EXPERIMENTAL BIOLOGY AND MEDICINE | 2017年 / 163卷 / 04期

关键词：

retinal pigment epithelium; ARPE-19; bFGF; neuronal differentiation; immunocytochemistry; ADULT HUMAN EYE; NEURAL RETINA; DIFFERENTIATION; TRANSDIFFERENTIATION; EXPRESSION; MULTIPOTENT; FENRETINIDE; PLASTICITY; ARPE-19; MARKERS;

D O I：

10.1007/s10517-017-3852-5

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

We studied the effect of bFGF on human retinal pigment epithelial cells in vitro In ARPE-19 cells, enhanced expression of KLF4 mRNA and reduced expression of PAX6, MITF, and OTX2 mRNA specific for retinal pigment epithelium were observed after bFGF application. The expression of KLF4 mRNA peaked in 72 h after bFGF application and then sharply decreased, which was accompanied by a 3-fold increase in TUBB3 mRNA expression (neuronal marker). Immunocytochemical analysis showed that in the presence of bFGF, some cells retained epithelial properties and showed positive staining for connexin-43, while others had long axon-like processes and demonstrated positive staining for beta III-tubulin, which attests to their neuronal transdifferentiation. Despite the prevalence of the epithelial properties, ARPE-19 cells under the influence of bFGF can show proneuronal properties.

引用

页码：574 / 582

页数：9

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