Photoactivatable fluorescent proteins

被引：359
作者：
Lukyanov, KA
Chudakov, DM
Lukyanov, S
Verkhusha, VV
机构：
[1] Russian Acad Sci, Inst Bioorgan Chem, Moscow 117997, Russia
[2] Univ Colorado, Dept Pharmacol, Aurora, CO 80045 USA
[3] Hlth Sci Ctr, Aurora, CO 80045 USA
来源：
NATURE REVIEWS MOLECULAR CELL BIOLOGY | 2005年 / 6卷 / 11期
关键词：
D O I：
10.1038/nrm1741
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
The fluorescence characteristics of photoactivatable proteins can be controlled by irradiating them with light of a specific wavelength, intensity and duration. This provides unique possibilities for the optical labelling and tracking of living cells, organelles and intracellular molecules in a spatio-temporal manner. Here, we discuss the properties of the available photoactivatable fluorescent proteins and their potential applications.
引用
收藏
页码：885 / 891
页数：7
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共 55 条
[41]   Improved monomeric red, orange and yellow fluorescent proteins derived from Discosoma sp red fluorescent protein [J].
Shaner, NC ;
Campbell, RE ;
Steinbach, PA ;
Giepmans, BNG ;
Palmer, AE ;
Tsien, RY .
NATURE BIOTECHNOLOGY, 2004, 22 (12) :1567-1572
[42]   Dynamics of single mRNPs in nuclei of living cells [J].
Shav-Tal, Y ;
Darzacq, X ;
Shenoy, SM ;
Fusco, D ;
Janicki, SM ;
Spector, DL ;
Singer, RH .
SCIENCE, 2004, 304 (5678) :1797-1800
[43]   A photochrornic acceptor as a reversible light-driven switch in fluorescence resonance energy transfer (FRET) [J].
Song, L ;
Jares-Erijman, EA ;
Jovin, TM .
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY A-CHEMISTRY, 2002, 150 (1-3) :177-185
[44]   Detection of snRNP assembly intermediates in Cajal bodies by fluorescence resonance energy transfer [J].
Stanek, D ;
Neugebauer, KM .
JOURNAL OF CELL BIOLOGY, 2004, 166 (07) :1015-1025
[45]   The green fluorescent protein [J].
Tsien, RY .
ANNUAL REVIEW OF BIOCHEMISTRY, 1998, 67 :509-544
[46]   Semi-rational engineering of a coral fluorescent protein into an efficient highlighter [J].
Tsutsui, H ;
Karasawa, S ;
Shimizu, H ;
Nukina, N ;
Miyawaki, A .
EMBO REPORTS, 2005, 6 (03) :233-238
[47]   Peripheral, non-centrosome-associated microtubules contribute to spindle formation in centrosome-containing cells [J].
Tulu, US ;
Rusan, NM ;
Wadsworth, P .
CURRENT BIOLOGY, 2003, 13 (21) :1894-1899
[48]   Phototransformation of green fluorescent protein with UV and visible light leads to decarboxylation of glutamate 222 [J].
van Thor, JJ ;
Gensch, T ;
Hellingwerf, KJ ;
Johnson, LN .
NATURE STRUCTURAL BIOLOGY, 2002, 9 (01) :37-41
[49]   The molecular properties and applications of Anthozoa fluorescent proteins and chromoproteins [J].
Verkhusha, VV ;
Lukyanov, KA .
NATURE BIOTECHNOLOGY, 2004, 22 (03) :289-296
[50]   Conversion of the monomeric red fluorescent protein into a photoactivatable probe [J].
Verkhusha, VV ;
Sorkin, A .
CHEMISTRY & BIOLOGY, 2005, 12 (03) :279-285
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