Photoactivatable fluorescent proteins

被引:359
作者
Lukyanov, KA
Chudakov, DM
Lukyanov, S
Verkhusha, VV
机构
[1] Russian Acad Sci, Inst Bioorgan Chem, Moscow 117997, Russia
[2] Univ Colorado, Dept Pharmacol, Aurora, CO 80045 USA
[3] Hlth Sci Ctr, Aurora, CO 80045 USA
关键词
D O I
10.1038/nrm1741
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
The fluorescence characteristics of photoactivatable proteins can be controlled by irradiating them with light of a specific wavelength, intensity and duration. This provides unique possibilities for the optical labelling and tracking of living cells, organelles and intracellular molecules in a spatio-temporal manner. Here, we discuss the properties of the available photoactivatable fluorescent proteins and their potential applications.
引用
收藏
页码:885 / 891
页数:7
相关论文
共 55 条
[1]   An optical marker based on the UV-induced green-to-red photoconversion of a fluorescent protein [J].
Ando, R ;
Hama, H ;
Yamamoto-Hino, M ;
Mizuno, H ;
Miyawaki, A .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2002, 99 (20) :12651-12656
[2]   Regulated fast nucleocytoplasmic shuttling observed by reversible protein highlighting [J].
Ando, R ;
Mizuno, H ;
Miyawaki, A .
SCIENCE, 2004, 306 (5700) :1370-1373
[3]   Frequent fusion and fission of plant mitochondria with unequal nucleoid distribution [J].
Arimura, S ;
Yamamoto, J ;
Aida, GP ;
Nakazono, M ;
Tsutsumi, N .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2004, 101 (20) :7805-7808
[4]   Light-driven decarboxylation of wild-type green fluorescent protein [J].
Bell, AF ;
Stoner-Ma, D ;
Wachter, RM ;
Tonge, PJ .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2003, 125 (23) :6919-6926
[5]   Seeing the wood through the trees: A review of techniques for distinguishing green fluorescent protein from endogenous autofluorescence [J].
Billinton, N ;
Knight, AW .
ANALYTICAL BIOCHEMISTRY, 2001, 291 (02) :175-197
[6]   Structural basis for dual excitation and photoisomerization of the Aequorea victoria green fluorescent protein [J].
Brejc, K ;
Sixma, TK ;
Kitts, PA ;
Kain, SR ;
Tsien, RY ;
Ormo, M ;
Remington, SJ .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1997, 94 (06) :2306-2311
[7]   A monomeric red fluorescent protein [J].
Campbell, RE ;
Tour, O ;
Palmer, AE ;
Steinbach, PA ;
Baird, GS ;
Zacharias, DA ;
Tsien, RY .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2002, 99 (12) :7877-7882
[8]   Ultra-fast excited state dynamics in green fluorescent protein: Multiple states and proton transfer [J].
Chattoraj, M ;
King, BA ;
Bublitz, GU ;
Boxer, SG .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1996, 93 (16) :8362-8367
[9]   Kindling fluorescent proteins for precise in vivo photolabeling [J].
Chudakov, DM ;
Belousov, VV ;
Zaraisky, AG ;
Novoselov, VV ;
Staroverov, DB ;
Zorov, DB ;
Lukyanov, S ;
Lukyanov, KA .
NATURE BIOTECHNOLOGY, 2003, 21 (02) :191-194
[10]   Photoswitchable cyan fluorescent protein for protein tracking [J].
Chudakov, DM ;
Verkhusha, VV ;
Staroverov, DB ;
Souslova, EA ;
Lukyanov, S ;
Lukyanov, KA .
NATURE BIOTECHNOLOGY, 2004, 22 (11) :1435-1439