Actin and microtubules drive differential aspects of planar cell polarity in multiciliated cells

被引:139
作者
Werner, Michael E. [1 ]
Hwang, Peter [1 ]
Huisman, Fawn [2 ]
Taborek, Peter [2 ]
Yu, Clare C. [2 ]
Mitchell, Brian J. [1 ]
机构
[1] Northwestern Univ, Feinberg Sch Med, Dept Cell & Mol Biol, Chicago, IL 60611 USA
[2] Univ Calif Irvine, Dept Phys & Astron, Irvine, CA 92697 USA
基金
美国国家卫生研究院;
关键词
CILIATED CELLS; MOTILE CILIA; EPITHELIAL-CELLS; QUAIL OVIDUCT; BASAL BODIES; IN-VIVO; CILIOGENESIS; MICROFILAMENTS; ORGANIZATION; POLARIZATION;
D O I
10.1083/jcb.201106110
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Planar cell polarization represents the ability of cells to orient within the plane of a tissue orthogonal to the apical basal axis. The proper polarized function of multiciliated cells requires the coordination of cilia spacing and cilia polarity as well as the timing of cilia beating during metachronal synchrony. The planar cell polarity pathway and hydrodynamic forces have been shown to instruct cilia polarity. In this paper, we show how intracellular effectors interpret polarity to organize cellular morphology in accordance with asymmetric cellular function. We observe that both cellular actin and microtubule networks undergo drastic reorganization, providing differential roles during the polarized organization of cilia. Using computational angular correlation analysis of cilia orientation, we report a graded cellular organization downstream of cell polarity cues. Actin dynamics are required for proper cilia spacing, global coordination of cilia polarity, and coordination of metachronic cilia beating, whereas cytoplasmic microtubule dynamics are required for local coordination of polarity between neighboring cilia.
引用
收藏
页码:19 / 26
页数:8
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