Add and Go: FRET Acceptor for Live-Cell Measurements Modulated by Externally Provided Ligand

被引：1

作者：

Gavrikov, Alexey S. ^{[1
]}

Bozhanova, Nina G. ^{[1
]}

Baranov, Mikhail S. ^{[1
,2
]}

Mishin, Alexander S. ^{[1
]}

机构：

[1] Russian Acad Sci, Shemyakin Ovchinnikov Inst Bioorgan Chem, Miklukho Maklay St 16-10, Moscow 117997, Russia

[2] Pirogov Russian Natl Res Med Univ, Ostrovitianov 1, Moscow 117997, Russia

来源：

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | 2022年 / 23卷 / 08期

关键词：

FRET; fluorogen-activating proteins; FAPs; FLUORESCENT PROTEIN;

D O I：

10.3390/ijms23084396

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A substantial number of genetically encoded fluorescent sensors rely on the changes in FRET efficiency between fluorescent cores, measured in ratiometric mode, with acceptor photobleaching or by changes in fluorescence lifetime. We report on a modulated FRET acceptor allowing for simplified one-channel FRET measurement based on a previously reported fluorogen-activating protein, DiB1. Upon the addition of the cell-permeable chromophore, the fluorescence of the donor-fluorescent protein mNeonGreen decreases, allowing for a simplified one-channel FRET measurement. The reported chemically modulated FRET acceptor is compatible with live-cell experiments and allows for prolonged time-lapse experiments with dynamic energy transfer evaluation.

引用

页数：10

共 30 条

[1] A Guide to Fluorescent Protein FRET Pairs [J].

Bajar, Bryce T. ;

Wang, Emily S. ;

Zhang, Shu ;

Lin, Michael Z. ;

Chu, Jun .

SENSORS, 2016, 16 (09)

[2] Improving brightness and photostability of green and red fluorescent proteins for live cell imaging and FRET reporting [J].

Bajar, Bryce T. ;

Wang, Emily S. ;

Lam, Amy J. ;

Kim, Bongjae B. ;

Jacobs, Conor L. ;

Howe, Elizabeth S. ;

Davidson, Michael W. ;

Lin, Michael Z. ;

Chu, Jun .

SCIENTIFIC REPORTS, 2016, 6

[3] Engineering of a fluorescent chemogenetic reporter with tunable color for advanced live-cell imaging [J].

Benaissa, Hela ;

Ounoughi, Karim ;

Aujard, Isabelle ;

Fischer, Evelyne ;

Goiame, Rosette ;

Nguyen, Julie ;

Tebo, Alison G. ;

Li, Chenge ;

Le Saux, Thomas ;

Bertolin, Giulia ;

Tramier, Marc ;

Danglot, Lydia ;

Pietrancosta, Nicolas ;

Morin, Xavier ;

Jullien, Ludovic ;

Gautier, Arnaud .

NATURE COMMUNICATIONS, 2021, 12 (01)

[4] Red-Shifted Aminated Derivatives of GFP Chromophore for Live-Cell Protein Labeling with Lipocalins [J].

Bozhanova, Nina G. ;

Baranov, Mikhail S. ;

Baleeva, Nadezhda S. ;

Gavrikov, Alexey S. ;

Mishin, Alexander S. .

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2018, 19 (12)

[5] Protein labeling for live cell fluorescence microscopy with a highly photostable renewable signal [J].

Bozhanova, Nina G. ;

Baranov, Mikhail S. ;

Klementieva, Natalia V. ;

Sarkisyan, Karen S. ;

Gavrikov, Alexey S. ;

Yampolsky, Ilia V. ;

Zagaynova, Elena V. ;

Lukyanov, Sergey A. ;

Lukyanov, Konstantin A. ;

Mishin, Alexander S. .

CHEMICAL SCIENCE, 2017, 8 (10) :7138-7142

[6] A One Pot, One Step, Precision Cloning Method with High Throughput Capability [J].

Engler, Carola ;

Kandzia, Romy ;

Marillonnet, Sylvestre .

PLOS ONE, 2008, 3 (11)

[7]

Engler C, 2011, METHODS MOL BIOL, V729, P167, DOI 10.1007/978-1-61779-065-2_11

[8] Golden Gate Shuffling: A One-Pot DNA Shuffling Method Based on Type IIs Restriction Enzymes [J].

Engler, Carola ;

Gruetzner, Ramona ;

Kandzia, Romy ;

Marillonnet, Sylvestre .

PLOS ONE, 2009, 4 (05)

[9] In vivo Study of the Histone Chaperone Activity of Nucleolin by FRAP [J].

Gaume, Xavier ;

Monier, Karine ;

Argoul, Francoise ;

Mongelard, Fabien ;

Bouvet, Philippe .

BIOCHEMISTRY RESEARCH INTERNATIONAL, 2011, 2011

[10] Diheteroarylethenes as thermally stable photoswitchable acceptors in photochromic fluorescence resonance energy transfer (pcFRET) [J].

Giordano, L ;

Jovin, TM ;

Irie, M ;

Jares-Erijman, EA .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2002, 124 (25) :7481-7489

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