Antioxidant and angiotensin-I converting enzyme inhibitory peptides from Hippocampus abdominalis

被引：22

作者：

Kim, Hyun-Soo ^{[1
]}

Je, Jun-Geon ^{[1
]}

Ryu, Bomi ^{[1
]}

Kang, Nalae ^{[2
]}

Fernando, I. P. Shanura ^{[1
]}

Jayawardena, Thilina U. ^{[1
]}

Sanjeewa, K. K. Asanka ^{[1
]}

Oh, Jae-Young ^{[1
]}

Lee, Tae-Gee ^{[3
]}

Jeon, You-Jin ^{[1
]}

机构：

[1] Jeju Natl Univ, Sch Marine Biomed Sci, Dept Marine Life Sci, Jeju 63243, South Korea

[2] Chonnam Natl Univ, Dept Marine Biofood Sci, Coll Fisheries & Ocean Sci, Kwangju 59626, South Korea

[3] Jeonnam Prov Coll, Dept Hotel Cuisine & Baking, Damyang 57337, South Korea

来源：

EUROPEAN FOOD RESEARCH AND TECHNOLOGY | 2019年 / 245卷 / 02期

关键词：

Hippocampus abdominalis; Sephadex G-10; Q-TOF mass; Antioxidant; Antihypertensive; Peptides; RADICAL-SCAVENGING ACTIVITIES; PROTEIN HYDROLYSATE; MOLECULAR DOCKING; PURIFICATION; IDENTIFICATION; SEAHORSES; SILICO; FOOD;

D O I：

10.1007/s00217-018-3179-0

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

Bioactive peptides isolated from edible marine sources have been used as nutraceuticals and functional foods. The present study focuses on the alcalase hydrolysate of Hippocampus abdominalis for the isolation of antioxidative and angiotensin-I converting enzyme (ACE) inhibitory peptides. Initially, the H. abdominalis alcalase hydrolysate (HA) was separated using ultrafiltration (MWCO=5kDa), and have obtained the fraction, HA-III (MW5kDa), with strong bioactivity. This was further separated using gel filtration chromatography (Sephadex G-10) and reverse-phase high-performance liquid chromatography (RP-HPLC). The active Sephadex G-10 fraction was analyzed by the Q-TOF mass spectrometry, nine peptides were identified within the molecular mass range between 757.8 and 990.1Da. Three peptides, GIIGPSGSP, IGTGIPGIW, and QIGFIW, showed strong ACE inhibitory activity and alkyl radical-scavenging activity. Molecular docking studies revealed that the ACE inhibitory activity of the three active peptides is mainly due to hydrogen bonding interactions and active site interactions between active peptides and ACE. This study demonstrated that the active peptides derived from H. abdominalis can be isolated and the proteolytic hydrolysates of it are potential antioxidant and antihypertensive agents.

引用

页码：479 / 487

页数：9

共 34 条

[1] In silico analysis and antihypertensive effect of ACE-inhibitory peptides from smooth-hound viscera protein hydrolysate: Enzyme-peptide interaction study using molecular docking simulation [J].

Abdelhedi, Ola ;

Nasri, Rim ;

Jridi, Mourad ;

Mora, Leticia ;

Oseguera-Toledo, Miguel Eduardo ;

Aristoy, Maria-Concepcion ;

Ben Amara, Ibtissem ;

Toldra, Fidel ;

Nasri, Moncef .

PROCESS BIOCHEMISTRY, 2017, 58 :145-159

[2] Purification and antioxidant properties of octapeptide from salmon byproduct protein hydrolysate by gastrointestinal digestion [J].

Ahn, Chang-Bum ;

Kim, Jeong-Gyun ;

Je, Jae-Young .

FOOD CHEMISTRY, 2014, 147 :78-83

[3] Contribution of Leu and Hyp residues to antioxidant and ACE-inhibitory activities of peptide sequences isolated from squid gelatin hydrolysate [J].

Aleman, A. ;

Gimenez, B. ;

Perez-Santin, E. ;

Gomez-Guillen, M. C. ;

Montero, P. .

FOOD CHEMISTRY, 2011, 125 (02) :334-341

[4]

Byun HG, 2002, J BIOCHEM MOL BIOL, V35, P239

[5] Active peptides from skate (Okamejei kenojei) skin gelatin diminish angiotensin-I converting enzyme activity and intracellular free radical-mediated oxidation [J].

Dai-Hung Ngo ;

Ryu, BoMi ;

Kim, Se-Kwon .

FOOD CHEMISTRY, 2014, 143 :246-255

[6] Production of angiotensin I converting enzyme inhibitory peptides from sea bream scales [J].

Fahmi, A ;

Morimura, S ;

Guo, HC ;

Shigematsu, T ;

Kida, K ;

Uemura, Y .

PROCESS BIOCHEMISTRY, 2004, 39 (10) :1195-1200

[7] Antioxidant activity of cod (Gadus morhua) protein hydrolysates: Fractionation and characterisation of peptide fractions [J].

Farvin, K. H. Sabeena ;

Andersen, Lisa Lystbaek ;

Otte, Jeanette ;

Nielsen, Henrik Hauch ;

Jessen, Flemming ;

Jacobsen, Charlotte .

FOOD CHEMISTRY, 2016, 204 :409-419

[8] Revalorisation of bovine collagen as a potential precursor of angiotensin 1-converting enzyme (ACE) inhibitory peptides based on in silico and in vitro protein digestions [J].

Fu, Yu ;

Young, Jette Feveile ;

Lokke, Mette Marie ;

Lametsch, Rene ;

Aluko, Rotimi E. ;

Therkildsen, Margrethe .

JOURNAL OF FUNCTIONAL FOODS, 2016, 24 :196-206

[9] Angiotensin I-converting enzyme inhibitory peptides from Sipuncula (Phascolosoma esculenta): Purification, identification, molecular docking and antihypertensive effects on spontaneously hypertensive rats [J].

Guo, Mingrong ;

Chen, Xujun ;

Wu, Yanling ;

Zhang, Lujia ;

Huang, Weixue ;

Yuan, Ying ;

Fang, Ming ;

Xie, Jingli ;

Wei, Dongzhi .

PROCESS BIOCHEMISTRY, 2017, 63 :84-95

[10] Two Novel Bioactive Peptides from Antarctic Krill with Dual Angiotensin Converting Enzyme and Dipeptidyl Peptidase IV Inhibitory Activities [J].

Ji, Wei ;

Zhang, Chaohua ;

Ji, Hongwu .

JOURNAL OF FOOD SCIENCE, 2017, 82 (07) :1742-1749

← 1 2 3 4 →