A new method for determination of apparent kinetics parameters in recirculating packed-bed immobilized enzyme reactors

被引：27

作者：

Özdural, AR ^{[1
]}

Tanyolaç, D

Demircan, Z

Boyaci, IH

Mutlu, M

Webb, C

机构：

[1] Hacettepe Univ, Dept Chem Engn, TR-06532 Ankara, Turkey

[2] Hacettepe Univ, Dept Food Engn, TR-06532 Ankara, Turkey

[3] UMIST, Dept Chem Engn, Satake Ctr Grain Proc Engn, Manchester M60 1QD, Lancs, England

来源：

CHEMICAL ENGINEERING SCIENCE | 2001年 / 56卷 / 11期

关键词：

enzyme; kinetics; packed-bed; recirculation reactor; immobilized glucose oxidase; modelling;

D O I：

10.1016/S0009-2509(01)00049-5

中图分类号：

TQ [化学工业];

学科分类号：

0817 ;

摘要：

In this study, a simple and effective technique for characterizing Michaelis-Menten apparent kinetic parameters in packed-bed immobilized enzyme reactors is presented. The apparent kinetic parameters of immobilized glucose oxidase on weak base ion exchanger resin (Duolite A 568) were determined for different substrate flow rates in a recirculation system and compared with those for soluble glucose oxidase. It was observed that, for the experimental conditions, the immobilized enzyme K-m' values in a packed-bed reactor were less than the soluble enzyme K-m value and were flow dependent. The value of K-m' decreased with increasing flow rate. (C) 2001 Elsevier Science Ltd. AH rights reserved.

引用

页码：3483 / 3490

页数：8

共 50 条

[41] Mathematical modelling of steady-state concentration in immobilized glucose isomerase of packed-bed reactors
V. Margret PonRani
L. Rajendran
Journal of Mathematical Chemistry, 2012, 50 : 1333 - 1346
[42] Comparison of ferric iron generation by different species of acidophilic bacteria immobilized in packed-bed reactors
Rowe, Owen F.
Johnson, D. Barrie
SYSTEMATIC AND APPLIED MICROBIOLOGY, 2008, 31 (01) : 68 - 77
[43] Innovations in packed-bed reactors utilizing immobilized lipase catalysts: A comprehensive technical and scientific review
Silva, Allison Ruan de Morais
Goncalves, Luciana Rocha Barros
da Silva Jr, Ivanildo Jose
MOLECULAR CATALYSIS, 2025, 573
[44] Continuous production of honey oligosaccharides in packed-bed reactors with immobilized ?-glucosidase from Metschnikowia reukaufii
Garcia-Gonzalez, Martin
Narmontaite, Egle
Cervantes, Fadia, V
Plou, Francisco J.
Betancor, Lorena
Fernandez-Lobato, Maria
BIOCATALYSIS AND AGRICULTURAL BIOTECHNOLOGY, 2023, 47
[45] Transversal Thermal Patterns in Packed-Bed Reactors with Simple Kinetics: Bifurcation Criterion and Simulations
Nekhamkina, Olga
Sheintuch, Moshe
AICHE JOURNAL, 2011, 57 (03) : 735 - 748
[46] Amperometric determination of L-malic acid in a flow injection analysis manifold using packed-bed enzyme reactors
Prodromidis, MI
TzouwaraKarayanni, SM
Karayannis, MI
Vadgama, P
Maines, A
ANALYST, 1996, 121 (04) : 435 - 439
[47] STABILITY ANALYSIS OF ADIABATIC PACKED-BED REACTORS VIA POPOVS FREQUENCY METHOD
HAN, CD
INDUSTRIAL & ENGINEERING CHEMISTRY FUNDAMENTALS, 1969, 8 (01): : 16 - &
[48] Kinetics of glucose oxidase immobilized in p(HEMA)-hydrogel microspheres in a packed-bed bioreactor
Brahim, S
Narinesingh, D
Guiseppi-Elie, A
JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC, 2002, 18 (1-3) : 69 - 80
[49] New Method of Rigorous Modeling and CFD Simulation for Methanol-Steam Reforming in Packed-Bed Reactors
Ziarati, Mahmoud
Roozbahani, Mohammad Ali Ghafouri
Khandan, Nahid
CHEMICAL ENGINEERING COMMUNICATIONS, 2016, 203 (10) : 1359 - 1373
[50] AUTOMATED ENZYME PACKED-BED SYSTEM FOR THE DETERMINATION OF VITAMIN-C IN FOODSTUFFS
DAILY, S
ARMFIELD, SJ
HAGGETT, BGD
DOWNS, MEA
ANALYST, 1991, 116 (06) : 569 - 572

← 1 2 3 4 5 →