A new method for determination of apparent kinetics parameters in recirculating packed-bed immobilized enzyme reactors

被引：27

作者：

Özdural, AR ^{[1
]}

Tanyolaç, D

Demircan, Z

Boyaci, IH

Mutlu, M

Webb, C

机构：

[1] Hacettepe Univ, Dept Chem Engn, TR-06532 Ankara, Turkey

[2] Hacettepe Univ, Dept Food Engn, TR-06532 Ankara, Turkey

[3] UMIST, Dept Chem Engn, Satake Ctr Grain Proc Engn, Manchester M60 1QD, Lancs, England

来源：

CHEMICAL ENGINEERING SCIENCE | 2001年 / 56卷 / 11期

关键词：

enzyme; kinetics; packed-bed; recirculation reactor; immobilized glucose oxidase; modelling;

D O I：

10.1016/S0009-2509(01)00049-5

中图分类号：

TQ [化学工业];

学科分类号：

0817 ;

摘要：

In this study, a simple and effective technique for characterizing Michaelis-Menten apparent kinetic parameters in packed-bed immobilized enzyme reactors is presented. The apparent kinetic parameters of immobilized glucose oxidase on weak base ion exchanger resin (Duolite A 568) were determined for different substrate flow rates in a recirculation system and compared with those for soluble glucose oxidase. It was observed that, for the experimental conditions, the immobilized enzyme K-m' values in a packed-bed reactor were less than the soluble enzyme K-m value and were flow dependent. The value of K-m' decreased with increasing flow rate. (C) 2001 Elsevier Science Ltd. AH rights reserved.

引用

页码：3483 / 3490

页数：8

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