Clusters of charged residues at the C terminus of MotA and N terminus of MotB are important for function of the Escherichia coli flagellar motor

被引：12

作者：

Hosking, Edan R. ^{[1
]}

Manson, Michael D. ^{[1
]}

机构：

[1] Texas A&M Univ, Dept Biol, College Stn, TX 77843 USA

来源：

JOURNAL OF BACTERIOLOGY | 2008年 / 190卷 / 15期

关键词：

D O I：

10.1128/JB.00407-08

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

MotA contains a conserved C-terminal cluster of negatively charged residues, and MotB contains a conserved N-terminal cluster of positively charged residues. Charge-altering mutations affecting these residues impair motility but do not diminish Mot protein levels. The motility defects are reversed by second-site mutations targeting the same or partner protein.

引用

页码：5517 / 5521

页数：5

共 23 条

[1] THE MOTA PROTEIN OF ESCHERICHIA-COLI IS A PROTON-CONDUCTING COMPONENT OF THE FLAGELLAR MOTOR [J].

BLAIR, DF ;

BERG, HC .

CELL, 1990, 60 (03) :439-449

[2] BACTERIAL MOTILITY - MEMBRANE TOPOLOGY OF THE ESCHERICHIA-COLI MOTB PROTEIN [J].

CHUN, SY ;

PARKINSON, JS .

SCIENCE, 1988, 239 (4837) :276-278

[3] GENE SEQUENCE AND PREDICTED AMINO-ACID-SEQUENCE OF THE MOTA PROTEIN, A MEMBRANE-ASSOCIATED PROTEIN REQUIRED FOR FLAGELLAR ROTATION IN ESCHERICHIA-COLI [J].

DEAN, GE ;

MACNAB, RM ;

STADER, J ;

MATSUMURA, P ;

BURKS, C .

JOURNAL OF BACTERIOLOGY, 1984, 159 (03) :991-999

[4]

DEMOT R, 1994, MOL MICROBIOL, V12, P333

[5] LOCALIZATION OF THE SALMONELLA-TYPHIMURIUM FLAGELLAR SWITCH PROTEIN FLIG TO THE CYTOPLASMIC M-RING FACE OF THE BASAL BODY [J].

FRANCIS, NR ;

IRIKURA, VM ;

YAMAGUCHI, S ;

DEROSIER, DJ ;

MACNAB, RM .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1992, 89 (14) :6304-6308

[6] Mutations in motB suppressible by changes in stator or rotor components of the bacterial flagellar motor [J].

Garza, AG ;

Biran, R ;

Wohlschlegel, JA ;

Manson, MD .

JOURNAL OF MOLECULAR BIOLOGY, 1996, 258 (02) :270-285

[7] Extragenic suppression of motA missense mutations of Escherichia coli [J].

Garza, AG ;

Bronstein, PA ;

Valdez, PA ;

HarrisHaller, LW ;

Manson, MD .

JOURNAL OF BACTERIOLOGY, 1996, 178 (21) :6116-6122

[8] MOTILITY PROTEIN INTERACTIONS IN THE BACTERIAL FLAGELLAR MOTOR [J].

GARZA, AG ;

HARRISHALLER, LW ;

STOEBNER, RA ;

MANSON, MD .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1995, 92 (06) :1970-1974

[9] PROTON PUMP IS A MOLECULAR ENGINE OF MOTILE BACTERIA [J].

GLAGOLEV, AN ;

SKULACHEV, VP .

NATURE, 1978, 272 (5650) :280-282

[10] PROPOSAL FOR A PEPTIDOGLYCAN-ASSOCIATING ALPHA-HELICAL MOTIF IN THE C-TERMINAL REGIONS OF SOME BACTERIAL CELL-SURFACE PROTEINS [J].

KOEBNIK, R .

MOLECULAR MICROBIOLOGY, 1995, 16 (06) :1269-1270

← 1 2 3 →