Analysis of cyclic pyrolysis products formed from amino acid monomer

被引:64
作者
Choi, Sung-Seen [1 ]
Ko, Ji-Eun [1 ]
机构
[1] Sejong Univ, Dept Chem, Seoul 143747, South Korea
关键词
Amino acids; Cyclic pyrolysis products; TMAH; Silica; CHROMATOGRAPHY-MASS-SPECTROMETRY; GAS-CHROMATOGRAPHY; MECHANISTIC CONSIDERATIONS; HUMIC SUBSTANCES; ORGANIC-MATTER; PROTEIN; THERMOCHEMOLYSIS; IDENTIFICATION; ENCAPSULATION; METHYLATION;
D O I
10.1016/j.chroma.2011.09.055
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Amino acid was mixed with silica and tetramethylammonium hydroxide (TMAH) to favor pyrolysis of amino acid monomer. The pyrolysis products formed from amino acid monomer were using GC/MS and GC. 20 amino acids of alanine, arginine, asparagine, aspartic acid, cysteine, glutamic acid, glutamine, glycine, histidine, isoleucine, leucine, lysine, methionine, phenylalanine, proline, serine, threonine, tryptophan, tyrosine, and valine were analyzed. The pyrolysis products were divided into cyclic and non-cyclic products. Among the 20 amino acids, arginine, asparagine, glutamic acid, glutamine, histidine, lysine, and phenylalanine generated cyclic pyrolysis products of the monomer. New cyclic pyrolysis products were formed by isolation of amino acid monomers. They commonly had polar side functional groups to 5-, 6-, or 7-membered ring structure. Arginine, asparagine, glutamic acid, glutamine, histidine, and phenylalanine generated only 5- or 6-membered ring products. However, lysine generated both 6- and 7-membered ring compounds. Variations of the relative intensities of the cyclic pyrolysis products with the pyrolysis temperature and amino acid concentration were also investigated. (C) 2011 Elsevier B.V. All rights reserved.
引用
收藏
页码:8443 / 8455
页数:13
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