p53 is important for the anti-invasion of ganoderic acid T in human carcinoma cells

被引：40
作者：
Chen, Nian-Hong [2 ]
Zhong, Jian-Jiang [1 ,2 ]
机构：
[1] Shanghai Jiao Tong Univ, Sch Life Sci & Biotechnol, Minist Educ, Key Lab Microbial Metab, Shanghai 200240, Peoples R China
[2] E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China
来源：
PHYTOMEDICINE | 2011年 / 18卷 / 8-9期
关键词：
Ganoderic acid T (GA-T); p53; Invasion; Matrix metalloproteinase-2/9 (MMP-2/9); Nuclear factor-kappa B (NF-kappa B); Urokinase-type plaminogen activator (uPA); WILD-TYPE P53; INHIBITS TUMOR INVASION; NITRIC-OXIDE SYNTHASE; BREAST-CANCER CELLS; FACTOR-KAPPA-B; IN-VITRO; EXPRESSION; GENE; LUCIDUM; ACTIVATION;
D O I：
10.1016/j.phymed.2011.01.011
中图分类号：
Q94 [植物学];
学科分类号：
071001 ;
摘要：
The function of p53 induced by ganoderic acids (GAs) in anti-invasion was unknown, although our previous work reported the inhibition of tumor invasion and metastais by Ganoderic acid T (GA-T). This work indicated that GA-T promoted cell aggregation, inhibited cell adhesion and surpressed cell migration with a dose-dependent manner in human colon tumor cell lines of HCT-116 p53(+/+) and p53(-/-). Furthermore, comparing the ratios of HCT-116 p53(+/+) and p53(-/-) cells, p53 modified GA-T inhibition of migration and adhesion and GA-T promotion of cell aggregation, and p53 also modified GA-T inhibition of NF-kappa B nuclear translocation, I kappa B alpha degradation, and down-regulation of urokinase-type plaminogen activator (uPA), matrix metalloproteinase-2/9 (MMP-2/9), inducible nitric oxide synthase (iNOS/NOS2) protein expression and inducible nitric oxide (NO) production. The results indicated that p53 played an important role in anti-invasion of GA-T in human carcinoma cells. p53 may be an important target for GA-T inhibiting human carcinoma cells anti-invasion. (C) 2011 Elsevier GmbH. All rights reserved.
引用
收藏
页码：719 / 725
页数：7
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