Practical Procedures for Improving Detection of Circulating miRNAs in Cardiovascular Diseases

被引：4

作者：

Chen, Chia-Chun ^{[1
,2
]}

Peng, Chen-Ching ^{[1
]}

Fan, Pei-Chun ^{[3
,4
]}

Chu, Pao-Hsien ^{[5
]}

Chang, Yu-Sun ^{[1
,6
]}

Chang, Chih-Hsiang ^{[3
,4
]}

机构：

[1] Chang Gung Univ, Mol Med Res Ctr, 259 Wen Hwa 1st Rd, Taoyuan 33302, Taiwan

[2] Chang Gung Mem Hosp Linkou, Dept Colorectal Surg, 5 Fusing St, Taoyuan 333, Taiwan

[3] Chang Gung Univ, Coll Med, Linkou Med Ctr, Dept Nephrol,Kidney Res Ctr,Chang Gung Mem Hosp, 5 Fusing St, Taoyuan 333, Taiwan

[4] Chang Gung Univ, Grad Inst Clin Med Sci, 259 Wen Hwa 1st Rd, Taoyuan 33302, Taiwan

[5] Chang Gung Univ, Chang Gung Mem Hosp, Coll Med, Dept Cardiol, 199 Tung Hwa North Rd, Taipei 105, Taiwan

[6] Chang Gung Univ, Grad Inst Biomed Sci, 259 Wen Hwa 1st Rd, Taoyuan 33302, Taiwan

来源：

JOURNAL OF CARDIOVASCULAR TRANSLATIONAL RESEARCH | 2020年 / 13卷 / 06期

关键词：

MicroRNA; Heparin; Heparinase; Hemolysis; Normalization; MICRORNAS; QUANTIFICATION; BIOMARKERS; PLASMA; EXPRESSION; DIAGNOSIS; HEPARIN; SERUM; RNAS;

D O I：

10.1007/s12265-020-10019-2

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Hemolysis has been known to affect the measurement of circulating biomarkers. In this study, clinically applicable procedures for microRNA (miRNA) detection in serum samples of acute myocardial infarction patients were established. The 89 samples from patients admitted to the coronary care unit were collected. These samples obtained from heparin-treated and untreated patients were subjected to heparinase digestion prior to miRNA measurements by multiplex RT-qPCR. The good reproducibility of miRNA detection after heparinase digestion (average R-2 = 0.97) indicated that this method can be used routinely for samples regardless of heparin medication. Additionally, the degree of hemolysis in these samples was highly related to the hemoglobin absorbance at 414 nm. Based on the hemoglobin absorbance, five hemolysis-associated miRNAs were identified in our data normalized with respect to both the spike-in control and the RNA amount in a given sample. Using these calibration procedures, miRNAs can be accurately quantified and identified for clinical samples.

引用

页码：977 / 987

页数：11

共 33 条

[1] Comparison and evaluation of RNA quantification methods using viral, prokaryotic, and eukaryotic RNA over a 104 concentration range [J].

Aranda, Roman ;

Dineen, Shauna M. ;

Craig, Rhonda L. ;

Guerrieri, Richard A. ;

Robertson, James M. .

ANALYTICAL BIOCHEMISTRY, 2009, 387 (01) :122-127

[2] Identification of Reference Genes for Relative Quantification of Circulating MicroRNAs in Bovine Serum [J].

Bae, In-Seon ;

Chung, Ki Yong ;

Yi, Jongmin ;

Kim, Tae Il ;

Choi, Hwa-Sik ;

Cho, Young-Moo ;

Choi, Inho ;

Kim, Sang Hoon .

PLOS ONE, 2015, 10 (03)

[3] MicroRNAs in Cardiovascular Disease [J].

Barwari, Temo ;

Joshi, Abhishek ;

Mayr, Manuel .

JOURNAL OF THE AMERICAN COLLEGE OF CARDIOLOGY, 2016, 68 (23) :2577-2584

[4] Assessing sample and miRNA profile quality in serum and plasma or other biofluids [J].

Blondal, Thorarinn ;

Nielsen, Soren Jensby ;

Baker, Adam ;

Andreasen, Ditte ;

Mouritzen, Peter ;

Teilum, Maria Wrang ;

Dahlsveen, Ina K. .

METHODS, 2013, 59 (01) :S1-S6

[5] Heparin Selectively Affects the Quantification of MicroRNAs in Human Blood Samples [J].

Boeckel, Jes-Niels ;

Thome, Claudia E. ;

Leistner, David ;

Zeiher, Andreas M. ;

Fichtlscherer, Stephan ;

Dimmeler, Stefanie .

CLINICAL CHEMISTRY, 2013, 59 (07) :1125-1127

[6] MicroRNA-223 and microRNA-92a in stool and plasma samples act as complementary biomarkers to increase colorectal cancer detection [J].

Chang, Pi-Yueh ;

Chen, Chia-Chun ;

Chang, Yu-Sun ;

Tsai, Wen-Sy ;

You, Jeng-Fu ;

Lin, Geng-Ping ;

Chen, Ting-Wen ;

Chen, Jinn-Shiun ;

Chan, Err-Cheng .

ONCOTARGET, 2016, 7 (09) :10663-10675

[7] Overcoming Heparin-Associated RT-qPCR Inhibition and Normalization Issues for microRNA Quantification in Patients with Acute Myocardial Infarction [J].

Coelho-Lima, Jose ;

Mohammed, Ashfaq ;

Cormack, Suzanne ;

Jones, Samuel ;

Das, Rajiv ;

Egred, Mohaned ;

Panahi, Pedram ;

Ali, Simi ;

Spyridopoulos, Ioakim .

THROMBOSIS AND HAEMOSTASIS, 2018, 118 (07) :1257-1269

[8] A circulating miRNA signature for early diagnosis of acute kidney injury following acute myocardial infarction [J].

Fan, Pei-Chun ;

Chen, Chia-Chun ;

Peng, Chen-Ching ;

Chang, Chih-Hsiang ;

Yang, Chia-Hung ;

Yang, Chi ;

Chu, Lichieh Julie ;

Chen, Yung-Chang ;

Yang, Chih-Wei ;

Chang, Yu-Sun ;

Chu, Pao-Hsien .

JOURNAL OF TRANSLATIONAL MEDICINE, 2019, 17

[9] MicroRNAs in acute kidney injury [J].

Fan, Pei-Chun ;

Chen, Chia-Chun ;

Chen, Yung-Chang ;

Chang, Yu-Sun ;

Chu, Pao-Hsien .

HUMAN GENOMICS, 2016, 10 :29

[10] Regulation of microRNA biogenesis [J].

Ha, Minju ;

Kim, V. Narry .

NATURE REVIEWS MOLECULAR CELL BIOLOGY, 2014, 15 (08) :509-524

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