Multi Wavelengths Fusion Profiling for Quality Evaluation of Donglingcao Tablets Combined with Electrochemical Oscillation Fingerprints and Antioxidant Activity Analysis

For a long time, Donglingcao tablets (DLCT) has been considered as a valuable traditional Chinese medicine (TCM) preparation. The five-wavelength fusion fingerprints (FWFFP) of 16 batches of DLCT, which avoided fluctuation of single wavelength evaluation, were established to analyze fingerprint similarity by ratio fingerprint quantitative method (RFQM). Furthermore, electrochemical oscillation fingerprints (ECOFP) of DLCT from different manufacturers were studied by Belousov Zhabotinskii (B-Z) oscillating system at constant temperature. In addition, intrinsic characteristics of DLCT from different manufacturers were determined by chemometric methods, and they can well distinguish samples of each manufacturer. Meanwhile, partial least squares (PLS) model was built to predict antioxidant activity of DLCT and to correlate with components in the fingerprint, which proved significant contribution of peaks 16-rosmarinic acid and 19-oridonin. The combination of FWFFP with antioxidant and ECOFP provided a new idea for identification and analysis of DLCT.