Trypanosoma cruzi epimastigotes express the ouabain- and vanadate-sensitive (Na++K+)ATPase activity

被引：0

作者：

Caruso-Neves, C

Einicker-Lamas, M

Chagas, C

Oliveira, MM

Vieyra, A

Lopes, AG ^{[1
]}

机构：

[1] Univ Fed Rio de Janeiro, Inst Biofis Carlos Chagas Filho, Lab Fisiol Renal, BR-21941 Rio De Janeiro, Brazil

[2] Univ Fed Rio de Janeiro, Inst Biofis Carlos Chagas Filho, Lab Biomembranas, BR-21941 Rio De Janeiro, Brazil

[3] Univ Fed Rio de Janeiro, Inst Ciencias Biomed, Dept Bioquim Med, Rio De Janeiro, Brazil

来源：

ZEITSCHRIFT FUR NATURFORSCHUNG C-A JOURNAL OF BIOSCIENCES | 1998年 / 53卷 / 11-12期

关键词：

(Na++K+)ATPase; Trypanosoma cruzi; ATPase; epimastigote; ouabain;

D O I：

暂无

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The presence of (Na++K+)ATPase activity in CL14 clone and NIH NTY strain of Trypanosoma cruzi epimastigotes is demonstrated. A Na+ plus K+ stimulated ATPase activity is found in both strains. The optimal Na+/K+ ratio is 5:1 and 9:1 in CL14 clone and NIH NTY strain, respectively. In both strains, vanadate completely inhibits the ouabain-sensitive ATPase activity indicating that it belongs to the P-type (E-1/E-2) family of ion-transporting ATPases. The I-50 for vanadate is 0.66 +/- 0.04 and 0.01 +/- 0.02 mu M in CL14 clone and NIH NTY strain, respectively. These data indicate that both strains of T. cruzi epimastigotes express the ouabain- and vanadate-sensitive (Na++K+)ATPase activity. On the other hand, the discrepancy between the parameters analyzed for the inhibitors suggests that they express different isoforms of this enzyme.

引用

页码：1049 / 1054

页数：6

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