Oxidized phosphatidylcholine induces migration of bone marrow-derived mesenchymal stem cells through Kruppel-like factor 4-dependent mechanism

被引：3

作者：

Shin, Sang Hun ^{[1
,2
]}

Song, Hae Young ^{[1
,2
]}

Kim, Min Young ^{[1
,2
]}

Do, Eun Kyung ^{[1
,2
]}

Lee, Jung Sub ^{[3
]}

Kim, Jae Ho ^{[1
,2
]}

机构：

[1] Pusan Natl Univ, Sch Med, Dept Physiol, Yangsan 626870, South Korea

[2] Pusan Natl Univ, Med Res Ctr Ischem Tissue Regenerat, Yangsan 626870, South Korea

[3] Pusan Natl Univ, Sch Med, Dept Orthopaed Surg, Pusan 602739, South Korea

来源：

MOLECULAR AND CELLULAR BIOCHEMISTRY | 2011年 / 352卷 / 1-2期

关键词：

POVPC; Platelet-activating factor; Kruppel-like factor 4; Mesenchymal stem cells; Migration; SMOOTH-MUSCLE-CELLS; ACTIVATING-FACTOR RECEPTOR; LOW-DENSITY-LIPOPROTEIN; STIMULATES MIGRATION; STROMAL CELLS; IN-VIVO; PHOSPHOLIPIDS; EXPRESSION; ATHEROSCLEROSIS; DIFFERENTIATION;

D O I：

10.1007/s11010-011-0745-1

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Platelet-activating factor (PAF; 1-O-alkyl-2-acetyl-sn-glycero-3-phosphorylcholine) and PAF-like oxidized phospholipids including 1-palmitoyl-2-oxovaleroyl-sn-glycero-3-phosphorylcholine (POVPC) are generated upon LDL oxidation. The aim of this study was to evaluate the question of whether POVPC can regulate migration of human bone marrow-derived stem cells (hBMSCs) and to characterize signaling mechanisms involved in the POVPC-induced cell migration. POVPC treatment resulted in dose- and time-dependent increase of hBMSCs migration. Treatment of cells with BN52021, a specific antagonist of PAF receptor, completely blocked cell migration induced by not only PAF but also POVPC. Silencing of endogenous PAF receptor expression using PAF receptor-specific small interfering RNA resulted in significant attenuation of cell migration induced by PAF or POVPC. Both PAF and POVPC induced expression of Kruppel-like factor 4 (KLF4) in hBMSCs. POVPC- or PAF-induced cell migration was abrogated by small interfering RNA-mediated depletion of endogenous KLF4. These results suggest that PAF receptor plays a pivotal role in POVPC-induced migration of human BMSCs through PAF receptor-mediated expression of KLF4.

引用

页码：109 / 115

页数：7

共 44 条

[1] The binding of oxidized low density lipoprotein to mouse CD36 is mediated in part by oxidized phospholipids that are associated with both the lipid and protein moieties of the lipoprotein [J].