Quality Control of Next-Generation Sequencing-Based HIV-1 Drug Resistance Data in Clinical Laboratory Information Systems Framework

被引：9

作者：

Capina, Rupert ^{[1
]}

Li, Katherine ^{[1
]}

Kearney, Levon ^{[2
]}

Vandamme, Anne-Mieke ^{[3
,4
]}

Harrigan, P. Richard ^{[5
]}

Van Laethem, Kristel ^{[3
,6
]}

机构：

[1] Publ Hlth Agcy Canada, JC Wilt Infect Dis Res Ctr, Natl HIV & Retrovirol Labs, Winnipeg, MB R3E 3L5, Canada

[2] Publ Hlth Agcy Canada, Natl Microbiol Lab, Sci Informat Serv, Winnipeg, MB R3E 3R2, Canada

[3] Katholieke Univ Leuven, Rega Inst Med Res, Dept Microbiol Immunol & Transplantat, B-3000 Leuven, Belgium

[4] Univ Nova Lisboa, Inst Higiene & Med Trop, Ctr Global Hlth & Trop Med, Unidade Microbiol, P-1349008 Lisbon, Portugal

[5] Univ British Columbia, Dept Med, Vancouver, BC V6T 1Z3, Canada

[6] Univ Hosp Leuven, AIDS Reference Lab, B-3000 Leuven, Belgium

来源：

VIRUSES-BASEL | 2020年 / 12卷 / 06期

关键词：

laboratory information systems; HIV-1 drug resistance; next-generation sequencing; quality control; quality management; TRANSMISSION; MANAGEMENT; CHART; RNA;

D O I：

10.3390/v12060645

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Next-generation sequencing (NGS) in HIV drug resistance (HIVDR) testing has the potential to improve both clinical and public health settings, however it challenges the normal operations of quality management systems to be more flexible due to its complexity, massive data generation, and rapidly evolving protocols. While guidelines for quality management in NGS data have previously been outlined, little guidance has been implemented for NGS-based HIVDR testing. This document summarizes quality control procedures for NGS-based HIVDR testing laboratories using a laboratory information systems (LIS) framework. Here, we focus in particular on the quality control measures applied on the final sequencing product aligned with the recommendations from the World Health Organization HIV Drug Resistance Laboratory Network.

引用

页数：16

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