Effect of liposome-encapsulated nisin and bacteriocin-like substance P34 on Listeria monocytogenes growth in Minas frescal cheese

被引：97
作者：
Malheiros, Patricia da Silva [1 ]
Sant'Anna, Voltaire [1 ]
Barbosa, Matheus de Souza [2 ]
Brandelli, Adriano [1 ]
Gombossy de Melo Franco, Bernadette Dora [2 ]
机构：
[1] Univ Fed Rio Grande do Sul, ICTA, Lab Bioquim & Microbiol Aplicada, BR-91540000 Porto Alegre, RS, Brazil
[2] Univ Sao Paulo, Fac Ciencias Farmaceut, Lab Microbiol Alimentos, Dept Alimentos & Nutr Expt, Sao Paulo, Brazil
来源：
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY | 2012年 / 156卷 / 03期
关键词：
Bacteriocin; Encapsulation; Cheese; Listeria monocytogenes; Nisin; Nanovesicle; ANTIMICROBIAL PEPTIDE P34; LACTOBACILLUS-ACIDOPHILUS; STABILITY; SIZE; PURIFICATION; PRESERVATION; INHIBITION; EFFICIENCY; EFFICACY; MILK;
D O I：
10.1016/j.ijfoodmicro.2012.04.004
中图分类号：
TS2 [食品工业];
学科分类号：
0832 ;
摘要：
The efficacy of liposome-encapsulated nisin and bacteriocin-like substance (BLS) P34 to control growth of Listeria monocytogenes in Minas frescal cheese was investigated. Nisin and BLS P34 were encapsulated in partially purified soybean phosphatidylcholine (PC-1) and PC-1-cholesterol (7:3) liposomes. PC-1 nanovesicles were previously characterized. PC-1-cholesterol encapsulated nisin and BLS P34 presented, respectively, 218 nm and 158 nm diameters, zeta potential of -64 mV and -53 mV, and entrapment efficiency of 88.9% and 100%. All treatments reduced the population of L monocytogenes compared to the control during 21 days of storage of Minas frescal cheese at 7 degrees C. However, nisin and BLS P34 encapsulated in PC-1-cholesterol liposomes were less efficient in controlling L monocytogenes growth in comparison with free and PC-1 liposome-encapsulated bacteriocins. The highest inhibitory effect was observed for nisin and BLS P34 encapsulated in PC-1 liposomes after 10 days of storage of the product The encapsulation of bacteriocins in liposomes of partially purified soybean phosphatidylcholine may be a promising technology for the control of food-borne pathogens in cheeses. (C) 2012 Elsevier B.V. All rights reserved.
引用
收藏
页码：272 / 277
页数：6
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