Mutagenic and genotoxic effects induced by PM0.5 of different Italian towns in human cells and bacteria: The MAPEC_LIFE study

被引：30

作者：

Bonetta, Sara ^{[1
]}

Bonetta, Silvia ^{[1
]}

Schiliro, Tiziana ^{[1
]}

Ceretti, Elisabetta ^{[2
]}

Feretti, Donatella ^{[2
]}

Covolo, Loredana ^{[2
]}

Vannini, Samuele ^{[3
]}

Villarini, Milena ^{[3
]}

Moretti, Massimo ^{[3
]}

Verani, Marco ^{[4
]}

Carducci, Annalaura ^{[4
]}

Bagordo, Francesco ^{[5
]}

De Donna, Antonella ^{[5
]}

Bonizzoni, Silvia ^{[6
]}

Bonetti, Alberto ^{[7
]}

Pignata, Cristina ^{[1
]}

Carraro, Elisabetta ^{[1
]}

Gelatti, Umberto ^{[2
]}

机构：

[1] Univ Torino, Dept Publ Hlth & Pediat, 94 Piazza Polonia, I-10126 Turin, Italy

[2] Univ Brescia, Dept Med & Surg Specialties, Radiol Sci & Publ Hlth, 11 Viale Europa, I-25123 Brescia, Italy

[3] Univ Perugia, Dept Pharmaceut Sci, Via Giochetto, I-06122 Perugia, Italy

[4] Univ Pisa, Dept Biol, 35-39 Via S Zeno, I-56127 Pisa, Italy

[5] Univ Salento, Dept Biol & Environm Sci & Technol, 165 Via Manteroni, I-73100 Lecce, Italy

[6] Comune Brescia, 1 Piazza Repubbl, I-25100 Brescia, Italy

[7] Ctr Serv Multisettoriale & Tecnol CSMT Gest Scarl, 45 Via Branze, I-25123 Brescia, Italy

来源：

ENVIRONMENTAL POLLUTION | 2019年 / 245卷

关键词：

PM0.5; Mutagenicity; Genotoxicity; PAHs; Nitro-PAHs; PARTICULATE AIR-POLLUTION; IN-VITRO; CHEMICAL-COMPOSITION; ULTRAFINE PARTICLES; SEASONAL-VARIATION; OXIDATIVE STRESS; URBAN PM2.5; MATTER; SIZE; FINE;

D O I：

10.1016/j.envpol.2018.11.017

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

Particular matter (PM) is considered an atmospheric pollutant that mostly affects human health. The finest fractions of PM (PM2.5 or less) play a major role in causing chronic diseases. The aim of this study was to investigate the genotoxic effects of PM0.5 collected in five Italian towns using different bioassays. The role of chemical composition on the genotoxicity induced was also evaluated. The present study was included in the multicentre MAPEC_LIFE project, which aimed to evaluate the associations between air pollution exposure and early biological effects in Italian children. PM10 samples were collected in 2 seasons (winter and spring) using a high-volume multistage cascade impactor. The results showed that PM0.5 represents a very high proportion of PM10 (range 1063%). PM0.5 organic extracts were chemically analysed (PAH(s), nitro-PAH(s)) and tested by the comet assay (A549 and BEAS-2B cells), MN test (A549 cells) and Ames test on Salmonella strains (TA100, TA98, TA98NR and YG1021). The highest concentrations of PAHs and nitro-PAHs in PM0.5 were observed in the Torino, Brescia and Pisa samples in winter. The Ames test showed low mutagenic activity. The highest net revertants/m(3) were observed in the Torino and Brescia samples (winter), and the mutagenic effect was associated with PM0.5 (p<0.01), PAH and nitro-PAH (p<0.05) concentrations. The YG1021 strain showed the highest sensitivity to PM0.5 samples. No genotoxic effect of PM0.5 extracts was observed using A549 cells except for some samples in winter (comet assay), while BEAS-2B cells showed light DNA damage in the Torino, Brescia and Pisa samples in winter, highlighting the higher sensitivity of BEAS-2B cells, which was consistent with the Ames test (p<0.01). The results obtained showed that it is important to further investigate the finest fractions of PM, which represent a relevant percentage of PM10, taking into account the chemical composition and the biological effects induced. (C) 2018 Elsevier Ltd. All rights reserved.

引用

页码：1124 / 1135

页数：12

共 72 条

[1]

Anderson JO, 2012, J MED TOXICOL, V8, P166, DOI 10.1007/s13181-011-0203-1

[2]

[Anonymous], STAT AMB 63 2015 QUA

[3]

[Anonymous], J NEUROINTERV SURG

[4]

[Anonymous], NEPA452R08004

[5]

[Anonymous], 2018, Technical Report, World Health Organization, Version 2

[6]

[Anonymous], 487 OECD

[7]

[Anonymous], RAPP STAT AMB NEL CO

[8] Lifestyles and socio-cultural factors among children aged 6-8 years from five Italian towns: the MAPEC_LIFE study cohort [J].

Bagordo, Francesco ;

De Donno, Antonella ;

Grassi, Tiziana ;

Guido, Marcello ;

Devoti, Gabriele ;

Ceretti, Elisabetta ;

Zani, Claudia ;

Feretti, Donatella ;

Villarini, Milena ;

Moretti, Massimo ;

Salvatori, Tania ;

Carducci, Annalaura ;

Verani, Marco ;

Casini, Beatrice ;

Bonetta, Sara ;

Carraro, Elisabetta ;

Schiliro, Tiziana ;

Bonizzoni, Silvia ;

Bonetti, Alberto ;

Gelatti, Umberto .

BMC PUBLIC HEALTH, 2017, 17

[9] Characterization of urban aerosol: seasonal variation of mutagenicity and genotoxicity of PM2.5, PM1 and semi-volatile organic compounds [J].

Bocchi, Clara ;

Bazzini, Cristina ;

Fontana, Federica ;

Pinto, Giancarlo ;

Martino, Anna ;

Cassoni, Francesca .

MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS, 2016, 809 :16-23

[10] DNA damage in A549 cells exposed to different extracts of PM2.5 from industrial, urban and highway sites [J].

Bonetta, Sa. ;

Gianotti, V. ;

Bonetta, Si. ;

Gosetti, F. ;

Oddone, M. ;

Gennaro, M. C. ;

Carraro, E. .

CHEMOSPHERE, 2009, 77 (07) :1030-1034

← 1 2 3 4 5 6 7 8 →