The anti-inflammatory effects of biomaterials cross-linked with genipin

被引：1
|
作者：
Song, YanFei [1 ]
Ding, ShanShan [1 ]
Cui, YuanLu [1 ]
Wang, QiangSong [1 ]
Ye, Lei [1 ]
Zhang, Ye [1 ]
机构：
[1] Tianjin Univ Tradit Chinese Med, Tianjin State Key Lab Modern Chinese Med, Tianjin 300193, Peoples R China
来源：
ADVANCED RESEARCH ON MATERIAL ENGINEERING, CHEMISTRY AND BIOINFORMATICS, PTS 1 AND 2 (MECB 2011) | 2011年 / 282-283卷
关键词：
biomaterials; genipin; glutaraldehyde; iNOS; RAW; 264.7; IN-VITRO; MACROPHAGES; EXPRESSION; GENE;
D O I：
10.4028/www.scientific.net/AMR.282-283.412
中图分类号：
TP39 [计算机的应用];
学科分类号：
081203 ; 0835 ;
摘要：
This study was aimed to investigate the anti-inflammatory effects of the chitosan/gelatin membrane cross-linked with genipin (GP). Fourier transform infrared spectrophotometry (FT-IR) was used to characterize the cross-linking reaction. The XTT assay was applied to evaluate the cytotoxicity of the chitosan/gelatin membrane with RAW264.7 cells. Production of nitric oxide (NO) was measured by the Griess colorimetric method. The gene expression levels of inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2) and IL-6 were detected by quantitative real-time reverse-transcription polymerase chain reaction (real-time RT-PCR). These results suggested that the anti-inflammatory effects of genipin cross-linked chitosan/gelatin membrane might be the results from modulating the NO/iNOS pathway and inhibiting the mRNA expression of COX-2, IL-6 in activated macrophages.
引用
收藏
页码：412 / 416
页数：5
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