Modelling amperometric enzyme electrode with substrate cyclic conversion

被引：30

作者：

Baronas, R

Kulys, J

Ivanauskas, F

机构：

[1] Vilnius State Univ, Fac Math & Informat, LT-2600 Vilnius, Lithuania

[2] Vilnius Gediminas Tech Univ, LT-2040 Vilnius, Lithuania

[3] Vilnius State Univ, Inst Math & Informat, LT-2600 Vilnius, Lithuania

来源：

BIOSENSORS & BIOELECTRONICS | 2004年 / 19卷 / 08期

关键词：

biosensor; amperometry; modelling; amplification;

D O I：

10.1016/j.bios.2003.08.022

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

A mathematical model of amperometric enzyme electrodes in which chemical amplification by cyclic substrate conversion takes place in a single enzyme membrane has been developed. The model is based on non-stationary diffusion equations containing a non-linear term related to Michaelis-Menten kinetic of the enzymatic reaction. The digital simulation was carried out using the finite difference technique. The influence of the substrate concentration, the maximal enzymatic rate as well as the membrane thickness on the biosensor response was investigated. The numerical experiments demonstrate significant (up to dozens of times) gain in biosensor sensitivity at low concentrations of substrate when the biosensor response is under diffusion control. (C) 2003 Elsevier B.V. All rights reserved.

引用

页码：915 / 922

页数：8

共 27 条

[21] ENZYME ELECTRODES WITH SUBSTRATE AND CO-ENZYME AMPLIFICATION [J].