Contribution of β-lactamase and efflux pump overproduction to tazobactam-piperacillin resistance in clinical isolates of Escherichia coli

被引:12
|
作者
Suzuki, Yuuki [1 ]
Sato, Toyotaka [1 ]
Fukushima, Yukari [2 ]
Nakajima, Chie [2 ,3 ]
Suzuki, Yasuhiko [2 ,3 ]
Takahashi, Satoshi [4 ,5 ]
Yokota, Shin-ichi [1 ]
机构
[1] Sapporo Med Univ, Sch Med, Dept Microbiol, Sapporo, Hokkaido, Japan
[2] Hokkaido Univ, Res Ctr Zoonosis Control, Div Bioresources, Sapporo, Hokkaido, Japan
[3] Hokkaido Univ, Global Inst Collaborat Res & Educ GI CoRE, Global Stn Zoonosis Control, Sapporo, Hokkaido, Japan
[4] Sapporo Med Univ Hosp, Div Lab Med, Sapporo, Hokkaido, Japan
[5] Sapporo Med Univ, Sch Med, Dept Infect Control & Lab Med, Sapporo, Hokkaido, Japan
关键词
Antimicrobial resistance; AcrAB; Extended-spectrum beta-lactamase; Tazobactam-piperacillin; GENES;
D O I
10.1016/j.ijantimicag.2020.105919
中图分类号
R51 [传染病];
学科分类号
100401 ;
摘要
Introduction: Tazobactam-piperacillin (TZP) is a mixture of a broad-spectrum penicillin and an irreversible beta-lactamase inhibitor. TZP is effective against Gram-negative bacteria that produce extended-spectrum beta-lactamases, and it is used as a first-line or second-line drug to treat serious infections. Methods: This study identified three TZP-resistant and two TZP-intermediate strains among 514 clinical isolates of Escherichia coli. Results: These five isolates possessed one or more beta-lactamase genes, bla(TEM-1), bla(CTX-M-2), bla(CTX-M-14), and/or bla(CMY-8). The expression levels of beta-lactamase genes and acrAB genes in the strains were examined by using real-time reverse transcription PCR. The total enzymatic piperacillin-degrading activity in cells was determined. Two TZP-resistance mechanisms were identified: hyperproduction of TEM-1 in the two resistant strains; and simultaneous high production of beta-lactamase and efflux pump AcrAB in the two TZP-intermediate isolates. The latter are an international high-risk clone O25b:H4-ST131-H30R. Conclusion: TZP resistance is still rare in clinical isolates of E. coli. However, resistance can develop on high production and/or combinations of known antimicrobial resistance mechanisms in different ways. (C) 2020 Elsevier B.V. and International Society of Chemotherapy. All rights reserved.
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页数:9
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