Identification, phylogeny and phylogeography of circumfenestrate cyst nematodes (Nematoda: Heteroderidae) as inferred from analysis of ITS-rDNA

被引:42
作者
Subbotin, Sergei A. [1 ,2 ,3 ]
Del Prado Vera, Ignacio Cid [4 ]
Mundo-Ocampo, Manuel [3 ]
Baldwin, James G. [3 ]
机构
[1] Calif Dept Food & Agr, Plant Pest Diagnost Ctr, Sacramento, CA 95832 USA
[2] Russian Acad Sci, AN Severtsov Inst Ecol & Evolut, Ctr Parasitol, Moscow 117071, Russia
[3] Univ Calif Riverside, Dept Nematol, Riverside, CA 92521 USA
[4] Colegio Postgrad, Montecillo 56230, Mexico
关键词
Betulodera; Cactodera; diagnostics; DIVA; Globodera; Globodera artemisiae; Globodera mexicana; Globodera millefolii; Globodera pallida; PCR-ITS-RFLP; DISPERSAL-VICARIANCE ANALYSIS; POTATO CYST; GLOBODERA-PALLIDA; RIBOSOMAL DNA; MOLECULAR-IDENTIFICATION; SOLANACEOUS PLANTS; GENETIC DIVERSITY; G-ARTEMISIAE; NEW-ZEALAND; POPULATIONS;
D O I
10.1163/138855410X552661
中图分类号
Q95 [动物学];
学科分类号
071002 ;
摘要
Some 134 ITS rRNA gene sequences for circumfenestrate cyst nematodes and two sequences for non-cyst nematodes of the family Heteroderidae, of which 46 were newly obtained, were analysed by phylogenetic and phylogeographic methods. Sequence and phylogenetic analysis combined with known morphological, biological and geographical data allowed the identification, amongst samples original to this study, of several belonging to known valid species as well as others that might be new species. The phylogenetic analysis revealed six major clades for circumfenestrate cyst nematodes: i) Globodera from South and North America; ii) Globodera from Europe, Asia, Africa and Oceania; iii) Paradolichodera; iv) Punctodera; v) Cactodera; and vi) Betulodera. Monophylies of Punctodera, Cactodera and Betulodera were highly supported. The Betulodera clade occupied a basal position on all trees. Phylogeographic analysis suggested a North American origin of Punctoderinae with possible further long distance dispersal to South America, Africa and other regions. Molecular data supported synonymisation of G. achilleae with G. millefolii and of G. hypolysi with G. artemisiae. PCR-RFLP diagnostic profiles for some Globodera and Cactodera species are given. Problems of diagnostics for Globodera species using PCR with specific primers are discussed.
引用
收藏
页码:805 / 824
页数:20
相关论文
共 75 条
[1]  
[Anonymous], TYLENCHIDA PARASITES
[2]  
[Anonymous], 1986, TYLENCHIDA PARASITES
[3]  
AYUB M, 2000, 28 TAG ARB NEM 1 2 M, P36
[4]   COMPARATIVE DETAILED MORPHOLOGY OF THE HETERODERINAE FILIPEV AND SCHUURMANS STEKHOVEN, 1941, SENSU LUC ET-AL (1988) - PHYLOGENETIC SYSTEMATICS AND REVISED CLASSIFICATION [J].
BALDWIN, JG ;
SCHOUEST, LP .
SYSTEMATIC PARASITOLOGY, 1990, 15 (02) :81-106
[5]   Inter and intra-specific genomic variability of the potato cyst nematodes Globodera pallida and G-rostochiensis from Europe and South America using RAPD-PCR [J].
Bendezu, IF ;
Evans, K ;
Burrows, PR ;
De Pomerai, D ;
Canto-Saenz, M .
NEMATOLOGICA, 1998, 44 (01) :49-61
[6]  
BERNARD E.C., 2010, J NEMATOLOG IN PRESS, V42
[7]   Comparison of British populations of potato cyst nematodes with populations from continental Europe and South America using RAPDs [J].
Blok, VC ;
Phillips, MS ;
Harrower, BE .
GENOME, 1997, 40 (03) :286-293
[8]  
Blok VC, 1998, J NEMATOL, V30, P262
[9]  
BLOK VC, 1995, FUND APPL NEMATOL, V18, P575
[10]  
BOSSIS M, 1993, FUND APPL NEMATOL, V16, P47