Mesophilic alcohol dehydrogenase behavior in imidazolium based ionic liquids

被引：14
|
作者：
Dabirmanesh, Bahareh [1 ]
Khajeh, Khosro [1 ]
Akbari, Jafar [2 ]
Falahati, Hanieh [3 ]
Daneshjoo, Somayeh [4 ]
Heydari, Akbar [2 ]
机构：
[1] Tarbiat Modares Univ, Dept Biochem, Fac Biol Sci, Tehran, Iran
[2] Tarbiat Modares Univ, Dept Chem, Fac Sci, Tehran, Iran
[3] Univ Tehran, Dept Biotechnol, Fac Sci, Tehran, Iran
[4] Islamic Azad Univ, Fac Sci, Dept Microbiol, Sci & Res Branch, Tehran, Iran
来源：
JOURNAL OF MOLECULAR LIQUIDS | 2011年 / 161卷 / 03期
关键词：
Activity; Alcohol dehydrogenase; Fluorescence; Ionic liquids; Stability; ANTARCTICA LIPASE-B; STABILITY; BIOCATALYSIS; ENZYMES; TEMPERATURE; PEROXIDASE; KINETICS;
D O I：
10.1016/j.molliq.2011.05.007
中图分类号：
O64 [物理化学（理论化学）、化学物理学];
学科分类号：
070304 ; 081704 ;
摘要：
The use of ionic liquids to replace organic solvents in biocatalytic processes has recently gained much attention. Despite the wide applications of oxidoreductases, there are few reports of their catalyzed reaction in ionic liquid. We have investigated the influence of four water miscible ionic liquids on the activity, stability and structure of the mesophilic alcohol dehydrogenase from yeast. Upon changes in ionic liquids concentration, both activity and stability of the enzyme were affected. As the concentration of ionic liquids increased, K-m increased while k(cat) decreased. Associated conformational changes caused by ILs (150 mM) were monitored using fluorescence technique. Finally, the effects of Its cations and anions on the enzyme activity and stability in aqueous IL mixtures were discussed. (C) 2011 Elsevier B.V. All rights reserved.
引用
收藏
页码：139 / 143
页数：5
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