Co-incorporation of Chinese milk vetch (Astragalus sinicus L.), rice straw, and biochar strengthens the mitigation of Cd uptake by rice (Oryza sativa L.)

Soil cadmium (Cd) contamination is becoming a widespread concern because of its threat to global ecosystem health and food security. Co-incorporation of Chinese milk vetch (MV) and rice straw (RS) is a common agricultural practice in Southern China; however, the effects of combining these two materials with biochar on Cd bioavailability remain unclear. This study investigated the effects of MV, RS, rape straw biochar (RB), iron-modified biochar (FB), and their combinations on Cd uptake by rice through incubation and field experiments. The results showed that compared with the control without material input (CK), MV + RS (MR), MV + RS + RB (MRRB), and MV + RS + FB (MRFB) considerably reduced the Cd concentration in brown rice by 61.20 %, 65.38 %, and 62.65 %, respectively. Further-more, the treatments increased the formation of iron-manganese plaque (IMP) at different growth stages; MRRB and MRFB exhibited the highest increase rates among the treatments. Quantitatively, the Fe plaque and Mn plaque were increased by 20.61 %-47.23 % and 80.18 %-172.74 %, respectively. Compared with CK, the MRRB and MRFB treat-ments reduced the soil available Cd by 35.09 %-54.45 % and 38.20 %-50.20 %, respectively, at all stages. This de-crease was substantially lower than that observed in the MV, RS, and MR treatments. Similar trends were observed in the incubation experiment. Additionally, the Community Bureau of Reference Sequential Extraction Analysis indi-cated that the MRRB and MRFB treatments converted the bioavailable Cd fractions into a stable form. Partial least squares path model and redundancy analysis revealed that pH was the major factor influencing Cd bioavailability. This study emphasized that the dual impact factors from the enhancement of Cd passivation capability and IMP formation jointly result in the reduction of Cd uptake by rice. Consequently, the co-incorporation of MV, RS, and biochar is promising for remediating Cd-contaminated paddy soils in Southern China.