β3 subunit of Na,K ATPase regulates T cell activation with no involvement of Na,K ATPase activity

被引：10

作者：

Chruewkamlow, Nuttapol ^{[1
]}

Pata, Supansa ^{[1
,2
]}

Mahasongkram, Kodchakorn ^{[1
]}

Laopajon, Witida ^{[1
]}

Kasinrerk, Watchara ^{[1
,2
]}

Chiampanichayakul, Sawitree ^{[3
]}

机构：

[1] Chiang Mai Univ, Fac Associated Med Sci, Div Clin Immunol, Dept Med Technol, Chiang Mai 50200, Thailand

[2] Chiang Mai Univ, Fac Associated Med Sci, Natl Sci & Technol Dev Agcy, Biomed Technol Res Ctr,Natl Ctr Genet Engn & Biot, Chiang Mai 50200, Thailand

[3] Chiang Mai Univ, Fac Associated Med Sci, Dept Med Technol, Div Clin Microscopy, Chiang Mai 50200, Thailand

来源：

IMMUNOBIOLOGY | 2015年 / 220卷 / 05期

关键词：

beta(3) subunit; CD298; Na; K ATPase; Monoclonal antibody; T cell activation; LYMPHOCYTE-ACTIVATION; ALPHA-SUBUNIT; NA+/K+-ATPASE; EXPRESSION; IDENTIFICATION; PROGRESSION; MECHANISM; ISOZYMES; GENE; NA;

D O I：

10.1016/j.imbio.2014.11.017

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Na,K ATPase plays an important role in the regulation of Na+ and K+ ions that are required for normal resting membrane potential and various cellular functions. Na,K ATPase is composed of two subunits, a and 13 subunits. Engagement of the 13 subunit by an agonistic monoclonal antibody (mAb) P-3E10 inhibited T cell activation and induced the G0/G1 cell cycle arrest. In addition, mAb P-3E10 decreased CD25 expression. The mAb P-3E10, however, did not inhibit the proliferation of cell lines and the phagocytosis activity of phagocytes, and did not interfere with the Na,K ATPase activity. These results indicate that mAb P-3E10 reacts to the beta subunit and, as a consequence, brings about the regulation of the T cell activation without disturbing the Na,K pump activity. By sequential immunoprecipitation, we demonstrated the expression of the beta 3 subunit free form apart from the a subunit. In this study, we propose that the beta 3 subunits of Na,K ATPase are expressed separately from the a subunit, and play a role in regulation of the immune response. (C) 2015 Elsevier GmbH. All rights reserved.

引用

页码：634 / 640

页数：7

共 29 条

[21] Identification of a novel gene of the X,K-ATPase β-subunit family that is predominantly expressed in skeletal and heart muscles [J].