Loss of G-protein-signaling modulator 2 accelerates proliferation of lung adenocarcinoma via EGFR signaling pathway

被引：16

作者：

Deng, Mingming ^{[1
,5
,6
]}

Liu, Bofang ^{[7
]}

Zhang, Zhe ^{[3
]}

Chen, Yang ^{[1
]}

Wang, Yizhe ^{[1
]}

Wang, Ximing ^{[1
]}

Lv, Qingjie ^{[3
]}

Yang, Xianghong ^{[3
]}

Hou, Kezuo ^{[2
]}

Che, Xiaofang ^{[2
]}

Qu, Xiujuan ^{[2
]}

Liu, Yunpeng ^{[2
]}

Zhang, Ye ^{[4
]}

Hu, Xuejun ^{[1
]}

机构：

[1] China Med Univ, Dept Resp & Infect Dis Geriatr, Hosp 1, 155 North Nanjing St, Shenyang 110001, Peoples R China

[2] China Med Univ, Dept Med Oncol, Hosp 1, Shenyang 110001, Peoples R China

[3] China Med Univ, Dept Pathol, Shengjing Hosp, Shenyang 110001, Peoples R China

[4] China Med Univ, Lab Canc Inst 1, Hosp 1, 155 North Nanjing St, Shenyang 110001, Peoples R China

[5] China Japan Friendship Hosp, Ctr Resp Med, Dept Pulm & Crit Care Med, Beijing, Peoples R China

[6] Chinese Acad Med Sci & Peking Union Med Coll, Peking Union Med Coll, Grad Sch, Beijing, Peoples R China

[7] Zhejiang Univ, Sir Run Run Shaw Hosp, Coll Med, Dept Med Oncol, Hangzhou 310000, Zhejiang, Peoples R China

来源：

INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY | 2020年 / 122卷

基金：

中国国家自然科学基金;

关键词：

GPSM2; NSCLC; Cell proliferation; EGFR; HEARING-LOSS; LGN; CANCER; EXPRESSION; BINDING; GROWTH; GPSM2; NUMA;

D O I：

10.1016/j.biocel.2020.105716

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

G-protein-signaling modulator 2 (GPSM2) belongs to a protein family that regulates activation of G proteins and plays an important role in mitotic spindle orientation. However, the role of GPSM2 in lung adenocarcinoma (LUAD) is still unclear. In this study, it was found that GPSM2 correlates with clinicopathological features and patient's prognosis in LUAD. Knocking down GPSM2 promoted LUAD cell proliferation in vitro and in vivo. Mechanistically, it was demonstrated that GPSM2 knockdown accelerates cell proliferation via the EGFR pathway. These results confirmed that GPSM2 played an important role in LUAD. Moreover, GPSM2, as an independent prognostic factor, may serve as a potential drug target and prognostic biomarker in LUAD.

引用

页数：7

共 28 条

[1]

[Anonymous], 2017, TUMOUR BIOL

[2] Comprehensive Analysis of Deafness Genes in Families with Autosomal Recessive Nonsyndromic Hearing Loss [J].

Atik, Tahir ;

Onay, Huseyin ;

Aykut, Ayca ;

Bademci, Guney ;

Kirazli, Tayfun ;

Tekin, Mustafa ;

Ozkinay, Ferda .

PLOS ONE, 2015, 10 (11)

[3] The G-protein regulatory (GPR) motif-containing Leu-Gly-Asn-enriched protein (LGN) and Giα3 influence cortical positioning of the mitotic spindle poles at metaphase in symmetrically dividing mammalian cells [J].

Blumer, Joe B. ;

Kuriyama, Ryoko ;

Gettys, Thomas W. ;

Lanier, Stephen M. .

EUROPEAN JOURNAL OF CELL BIOLOGY, 2006, 85 (12) :1233-1240

[4] Concomitant binding of Afadin to LGN and F-actin directs planar spindle orientation [J].

Carminati, Manuel ;

Gallini, Sara ;

Pirovano, Laura ;

Alfieri, Andrea ;

Bisi, Sara ;

Mapelli, Marina .

NATURE STRUCTURAL & MOLECULAR BIOLOGY, 2016, 23 (02) :155-+

[5] Insc:LGN tetramers promote asymmetric divisions of mammary stem cells [J].

Culurgioni, Simone ;

Mari, Sara ;

Bonetti, Paola ;

Gallini, Sara ;

Bonetto, Greta ;

Brennich, Martha ;

Round, Adam ;

Nicassio, Francesco ;

Mapelli, Marina .

NATURE COMMUNICATIONS, 2018, 9

[6] G-protein-signaling modulator 2 expression and role in a CD133+ pancreatic cancer stem cell subset [J].

Dang, Sheng-Chun ;

Qian, Xiao-Bao ;

Jin, Wei ;

Cui, Lei ;

Chen, Ji-Xiang ;

Gu, Min .

ONCOTARGETS AND THERAPY, 2019, 12 :785-794

[7] β-Elemene inhibits peritoneal metastasis of gastric cancer cells by modulating FAK/Claudin-1 signaling [J].

Deng, Mingming ;

Zhang, Ye ;

Liu, Bofang ;

Chen, Yang ;

Song, Huicong ;

Yu, Ruoxi ;

Che, Xiaofang ;

Qu, Xiujuan ;

Liu, Yunpeng ;

Hu, Xuejun ;

Xu, Xiuying .

PHYTOTHERAPY RESEARCH, 2019, 33 (09) :2448-2456

[8] Limb-Bud and Heart Attenuates Growth and Invasion of Human Lung Adenocarcinoma Cells and Predicts Survival Outcome [J].

Deng, Mingming ;

Yu, Ruoxi ;

Wang, Shuo ;

Zhang, Ye ;

Li, Zhi ;

Song, Huicong ;

Liu, Bofang ;

Xu, Ling ;

Wang, Ximing ;

Zhang, Zhe ;

Lv, Qingjie ;

Wang, Xiaonan ;

Che, Xiaofang ;

Qu, Xiujuan ;

Liu, Yunpeng ;

Hu, Xuejun .

CELLULAR PHYSIOLOGY AND BIOCHEMISTRY, 2018, 47 (01) :223-234

[9] GPSM2 Mutations Cause the Brain Malformations and Hearing Loss in Chudley-McCullough Syndrome [J].

Doherty, Dan ;

Chudley, Albert E. ;

Coghlan, Gail ;

Ishak, Gisele E. ;

Innes, A. Micheil ;

Lemire, Edmond G. ;

Rogers, R. Curtis ;

Mhanni, Aizeddin A. ;

Phelps, Ian G. ;

Jones, Steven J. M. ;

Zhan, Shing H. ;

Fejes, Anthony P. ;

Shahin, Hashem ;

Kanaan, Moien ;

Akay, Hatice ;

Tekin, Mustafa ;

Triggs-Raine, Barbara ;

Zelinski, Teresa .

AMERICAN JOURNAL OF HUMAN GENETICS, 2012, 90 (06) :1088-1093

[10] A mammalian Partner of inscuteable binds NuMA and regulates mitotic spindle organization [J].

Du, QS ;

Stukenberg, PT ;

Macara, IG .

NATURE CELL BIOLOGY, 2001, 3 (12) :1069-1075

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