Simultaneous determination of 2-methoxyphenol, 2-methoxy-4-methylphenol, 2,6-dimethoxyphenol and 4′-hydroxy-3′-methoxyacetophenone in urine by capillary gas chromatography

被引：11

作者：

Bieniek, G ^{[1
]}

机构：

[1] Med Univ Silesia, Dept Mol Biol Biochem & Biopharm, PL-41200 Sosnowiec, Poland

来源：

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2003年 / 795卷 / 02期

关键词：

2-methoxyphenol; 2-methoxy-4-methylphenol; 2,6-dimethoxyphenol; 4 '-hydroxy-3 '-methoxyacetophenone;

D O I：

10.1016/S1570-0232(03)00593-2

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A method for the simultaneous determination of 2-methoxyphenol, 2-methoxy-4-methylphenol, 2,6-dimethoxyphenol and 4'-hydroxy-3'-methoxyacetophenone in urine has been described. The metabolites were analyzed after enzymatic hydrolysis and extraction on octyl (C8) cartridges by using gas chromatography with flame ionization detection and a 5/95% copolymer of diphenyl-poly(dimethylsiloxane) capillary column. Methoxyphenols were well separated within 12 min. Recovery was over 90% in the range from 0.5 to 20 mug/ml; the detection limit was varying in the range of 0.05-0.11 Rg/ml. The relative standard deviations and the accuracy were in the range of 3.1-15.5 and 2.4-16.0%, respectively. (C) 2003 Elsevier B.V. All rights reserved.

引用

页码：389 / 394

页数：6

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