Ammonia-oxidizing bacterial community and nitrification rates in constructed wetlands treating swine wastewater

被引：41
作者：
Dong, Xiuli [1 ]
Reddy, Gudigopuram B. [1 ]
机构：
[1] N Carolina Agr & Tech State Univ, Dept Nat Resources & Environm Design, Greensboro, NC 27411 USA
来源：
ECOLOGICAL ENGINEERING | 2012年 / 40卷
关键词：
Constructed wetland; Nitrification; Ammonia oxidizing bacteria; amoA gene; Bacterial diversity; 16S RIBOSOMAL-RNA; NITRIFYING BACTERIA; PCR; AMOA; PROTEOBACTERIA; DIVERSITY; ABUNDANCE; KINETICS; CULTURES; ARCHAEA;
D O I：
10.1016/j.ecoleng.2011.12.022
中图分类号：
Q14 [生态学（生物生态学）];
学科分类号：
071012 ; 0713 ;
摘要：
The goal of this study was to investigate the correlation between the potential nitrification (PN) rate and ammonia-oxidizing bacteria (AOB) in wetlands treated with swine wastewater. The wetlands showed high efficiency in the removal of NH4+, NO3-, and chemical oxygen demand (COD). Nitrification and denitrification occurred concurrently in the wetlands. The most probable number (MPN) value of AOB was highly related to NH4+ (R-2 = 0.92, P<0.05) and NO3- concentrations (R-2 = 0.96, P<0.05), but not significantly correlated with COD. The AOB Shannon-Weaver diversity index (H) and richness (S) which were calculated from digitalized profiles of denaturing gradient gel electrophoresis (DGGE) did not correlate with NH4+ and NO3- concentrations. The PN rates in detritus and soil samples highly correlated with NH4+ (R-2=0.92, P<0.5). The phylogenetic trees based on 16S rRNA gene sequences and amino-acid-residue sequences of the amoA gene showed that parts of the AOB from the wetlands were affiliated with beta-proteobacteria cluster 2, cluster 6a, cluster 7, and Nitrosospira sp. NSP65. (c) 2011 Elsevier B.V. All rights reserved.
引用
收藏
页码：189 / 197
页数：9
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