Multiple norovirus genotypes characterised from an oyster-associated outbreak of gastroenteritis

被引:74
作者
Gallimore, CI [1 ]
Cheesbrough, JS
Lamden, K
Bingham, C
Graya, JJ
Gray, JJ
机构
[1] Cent Publ Hlth Lab, Specialist & Ref Microbiol Div, Enter Resp & Neurol Virus Lab, Hlth Protect Agcy Ctr Infect, London NW9 5HT, England
[2] Royal Preston Hosp, Lancashire Teaching Hosp NHS Trust, Microbiol Serv, Preston, Lancs, England
[3] Cumbria & Lancashire Hlth Protect Unit, Preston, Lancs, England
[4] Chorley Borough Council, Chorley, England
关键词
norovirus; multiple strains; oysters; recombinants;
D O I
10.1016/j.ijfoodmicro.2005.02.003
中图分类号
TS2 [食品工业];
学科分类号
0832 ;
摘要
The diversity of norovirus (NV) genotypes was investigated in persons who were ill with acute gastroenteritis associated with the consumption of oysters. Initial results from a commercial enzyme immunoassay (EIA) indicated a mixed NV genogroup I (Gl) and II (GII) outbreak. A reverse-transcriptase (RT)-PCR for NVs was applied to nucleic acid extracted from faecal specimens collected from symptomatic cases. Using primers that amplified contiguous sequences in the ORF1/2 region of the NV genome and a hemi-nested PCR derived from this assay, three different GII and two GI NV genotypes were detected and the strains were characterised by DNA sequencing. Using this approach a recombinant NV genotype, rGII-3a (recombinant Harrow/Mexico) the predominant strain identified in several symptomatic cases from the outbreak, was detected and characterised. No other gastroenteric viruses, including rotavirus, astrovirus, sapovirus and adenovirus 40/41 were detected by RT-PCR and PCR. (c) 2005 Elsevier B.V. All rights reserved.
引用
收藏
页码:323 / 330
页数:8
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