Enantioselective production of levofloxacin by immobilized porcine liver esterase

被引：9

作者：

Lee, SY

Min, BH

Hwang, SH

Koo, YM

Lee, CK

Song, SW

Oh, SY

Lim, SM

Kim, SL

Kim, DI ^{[1
]}

机构：

[1] Inha Univ, Dept Biol Engn, Inchon 402751, South Korea

[2] Boryung Pharmaceut Co, Cent Res Inst, Ansan 425120, South Korea

来源：

BIOTECHNOLOGY LETTERS | 2001年 / 23卷 / 13期

关键词：

immobilized enzyme; levofloxacin; ofloxacin; porcine liver esterase;

D O I：

10.1023/A:1010554118301

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Porcine liver esterase, which cleaves ofloxacin butyl ester enantioselectively to levofloxacin, was successfully immobilized in calcium alginate and polyacrylamide gel. Immobilized esterase in 5% (w/v) calcium alginate exhibited 58% immobilization efficiency and could be reused five times without severe loss of enzyme activity. On the other hand, entrapped esterase in polyacrylamide gel, composed of 20% of total monomer and 8.3% of cross-linking agent, could be reused 10 times, and 51% of enzyme activity remained after the 10th batch without decrease of enantioselectivity. Compared with entrapped methods, significant reduction of enzyme activity was found in the case of physical adsorption on to QAE-Sephadex.

引用

页码：1033 / 1037

页数：5

共 17 条

[1] Enhancing enzymatic properties by the immobilization method [J].