Strategy for Modular Tagged High-Throughput Amplicon Sequencing

被引：23

作者：

de Carcer, Daniel Aguirre ^{[1
]}

Denman, Stuart E. ^{[1
]}

McSweeney, Chris ^{[1
]}

Morrison, Mark ^{[1
]}

机构：

[1] CSIRO Preventat Hlth Natl Res Flagship & Livestoc, Queensland Biosci Precinct, St Lucia, Qld 4067, Australia

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2011年 / 77卷 / 17期

关键词：

BACTERIA; FLORA;

D O I：

10.1128/AEM.05146-11

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The use and validation of a strategy that allows a universal set of bar-coded sequencing primers to be appended to an amplified PCR product is described. The strategy allows a modular approach, in that the same bar code can be used with two or more target-specific primer sets, even simultaneously.

引用

页码：6310 / 6312

页数：3

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