A genome-scale shRNA resource for transgenic RNAi in Drosophila

被引：1

作者：

Ni, Jian-Quan ^{[1
]}

Zhou, Rui ^{[1
]}

Czech, Benjamin ^{[2
]}

Liu, Lu-Ping ^{[1
]}

Holderbaum, Laura ^{[1
]}

Yang-Zhou, Donghui ^{[1
]}

Shim, Hye-Seok ^{[1
]}

Tao, Rong ^{[1
]}

Handler, Dominik ^{[4
]}

Karpowicz, Phillip ^{[1
]}

Binari, Richard ^{[1
]}

Booker, Matthew ^{[1
]}

Brennecke, Julius ^{[4
]}

Perkins, Lizabeth A. ^{[3
]}

Hannon, Gregory J. ^{[2
]}

Perrimon, Norbert ^{[1
]}

机构：

[1] Harvard Univ, Sch Med, Howard Hughes Med Inst, Dept Genet, Boston, MA 02115 USA

[2] Cold Spring Harbor Lab, Howard Hughes Med Inst, Watson Sch Biol Sci, Cold Spring Harbor, NY 11724 USA

[3] Harvard Univ, Massachusetts Gen Hosp, Sch Med, Pediat Surg Res Labs, Boston, MA USA

[4] Inst Mol Biotechnol, Vienna, Austria

来源：

NATURE METHODS | 2011年 / 8卷 / 05期

基金：

欧洲研究理事会; 奥地利科学基金会;

关键词：

GENE-EXPRESSION; MELANOGASTER; PATHWAY; GERMLINE;

D O I：

10.1038/NMETH.1592

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Existing transgenic RNAi resources in Drosophila melanogaster based on long double-stranded hairpin RNAs are powerful tools for functional studies, but they are ineffective in gene knockdown during oogenesis, an important model system for the study of many biological questions. We show that shRNAs, modeled on an endogenous microRNA, are extremely effective at silencing gene expression during oogenesis. We also describe our progress toward building a genome-wide shRNA resource.

引用

页码：405 / U46

页数：5

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